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ZFIN ID: ZDB-FIG-131105-18
Rosenfeld et al., 2013 - Small heat shock proteins Hspb7 and Hspb12 regulate early steps of cardiac morphogenesis. Developmental Biology   381(2):389-400 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Fig. 3

Hspb12 is required for normal numbers of ventricular cardiomyocytes. (A) Representative cardiac phenotypes observed at 48 hpf development in embryos derived from the myl7:gfp; myl7:dsRed-nls reporter strain stained with S46 antibody to differentiate the atrium (blue) from the ventricle (green). (B) Quantification of cardiomyocytes in wildtype embryos (wt, n=7) or embryos injected with standard control MO (control, n=7), hspb7 MO (hspb7, n=7), hspb12 MO (hspb12, n=8), or both hspb7 and hspb12 MOs (hspb7+12, n=6). Np<0.05 by Student′s t-test in comparison to standard control group. The bars represent 10 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed f2Tg; twu34Tg control Long-pec cardiac muscle cell IFL
f2Tg; twu34Tg standard conditions Long-pec cardiac muscle cell IFL
f2Tg; twu34Tg + MO1-hspb12 standard conditions Long-pec cardiac muscle cell IFL
f2Tg; twu34Tg + MO1-hspb12 + MO1-hspb7 standard conditions Long-pec cardiac muscle cell IFL
f2Tg; twu34Tg + MO1-hspb7 standard conditions Long-pec cardiac muscle cell IFL
EGFP f2Tg; twu34Tg control Long-pec cardiac muscle cell IFL
f2Tg; twu34Tg standard conditions Long-pec cardiac muscle cell IFL
f2Tg; twu34Tg + MO1-hspb12 standard conditions Long-pec cardiac muscle cell IFL
f2Tg; twu34Tg + MO1-hspb12 + MO1-hspb7 standard conditions Long-pec cardiac muscle cell IFL
f2Tg; twu34Tg + MO1-hspb7 standard conditions Long-pec cardiac muscle cell IFL
myh6 Ab-S46 f2Tg; twu34Tg control Long-pec atrial myocardium cardiac muscle cell IHC
Ab-S46 f2Tg; twu34Tg standard conditions Long-pec atrial myocardium cardiac muscle cell IHC
Ab-S46 f2Tg; twu34Tg + MO1-hspb12 standard conditions Long-pec atrial myocardium cardiac muscle cell IHC
Ab-S46 f2Tg; twu34Tg + MO1-hspb12 + MO1-hspb7 standard conditions Long-pec atrial myocardium cardiac muscle cell IHC
Ab-S46 f2Tg; twu34Tg + MO1-hspb7 standard conditions Long-pec atrial myocardium cardiac muscle cell IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
f2Tg; twu34Tg + MO1-hspb12 standard conditions Long-pec atrial myocardium cardiac muscle cell normal amount, normal
Long-pec heart morphology, abnormal
Long-pec ventricular myocardium cardiac muscle cell decreased amount, abnormal
f2Tg; twu34Tg + MO1-hspb12 + MO1-hspb7 standard conditions Long-pec atrial myocardium cardiac muscle cell normal amount, normal
Long-pec heart morphology, abnormal
Long-pec ventricular myocardium cardiac muscle cell decreased amount, abnormal
f2Tg; twu34Tg + MO1-hspb7 standard conditions Long-pec cardiac muscle cell normal amount, normal
Long-pec heart morphology, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 381(2), Rosenfeld, G.E., Mercer, E.J., Mason, C.E., and Evans, T., Small heat shock proteins Hspb7 and Hspb12 regulate early steps of cardiac morphogenesis, 389-400, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.