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ZFIN ID: ZDB-FIG-131105-17
Rosenfeld et al., 2013 - Small heat shock proteins Hspb7 and Hspb12 regulate early steps of cardiac morphogenesis. Developmental Biology   381(2):389-400 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 2

Cardiac small heat shock proteins are required for morphogenesis of the heart tube and they interact genetically with gata4. (A) Representative cardiac phenotypes observed at 3 dpf of myl7:gfp embryos. Non-looping is defined by the failure of the ventricle to align perpendicular to the atrium. (B) Quantification of the cardiac phenotypes in wildtype embryos (wt, n=54), embryos injected with standard control MO (control, n=46), hspb7 MO (hspb7, n=67), or hspb12 MO (hspb12, n=89). (C) Quantification of the cardiac phenotypes in wildtype embryos (wt, n=20) or embryos injected with sub-threshold concentrations of MO specific for gata4 (n=38), hspb7 (n=36), or hspb12 (n=29), or with the same concentrations combined (gata4+hspb7, n=29) or (gata4+hspb12, n=49).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP twu34Tg control Protruding-mouth heart IFL
twu34Tg + MO1-hspb12 standard conditions Protruding-mouth heart IFL
twu34Tg + MO1-hspb7 standard conditions Protruding-mouth heart IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
twu34Tg + MO1-hspb12 standard conditions Protruding-mouth heart morphology, abnormal
Protruding-mouth heart looping disrupted, abnormal
twu34Tg + MO1-hspb7 standard conditions Protruding-mouth heart morphology, abnormal
Protruding-mouth heart looping disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 381(2), Rosenfeld, G.E., Mercer, E.J., Mason, C.E., and Evans, T., Small heat shock proteins Hspb7 and Hspb12 regulate early steps of cardiac morphogenesis, 389-400, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.