FIGURE

Fig. S7

ID

ZDB-FIG-131105-30

Publication

Rosenfeld et al., 2013 - Small heat shock proteins Hspb7 and Hspb12 regulate early steps of cardiac morphogenesis

Other Figures

All Figure Page

Back to All Figure Page

Fig. S7

The hspb7 translation-blocker MO and hspb7 splicing-blocker MO synergize to cause jogging defects when used together at sub-threshold levels. A) Representative jogging phenotypes at 30hpf in embryos derived from the myl7:gfp reporter strain. B) Quantification of jogging phenotypes in wildtype embryos (wt, n=32), or embryos injected with the standard control MO (control, n=28), hspb7 7MO at 0.15mM (ssMO, n=81), hspb7 7MO1 at 0.075 mM (tbMO, n=66), 7MO1 + standard control (hsbp7 tbMO + control, n=82), or 7MO + 7MO1 (hspb7 tbMO + ssMO, n=78). The 7MO splice-blocker MO used at 0.15mM does not generate the left-right defects observed at the higher concentrations but does so when co-injected with a sub-threshold concentration of the translation blocker MO.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 381(2), Rosenfeld, G.E., Mercer, E.J., Mason, C.E., and Evans, T., Small heat shock proteins Hspb7 and Hspb12 regulate early steps of cardiac morphogenesis, 389-400, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.