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ZIRC
ZFIN ID: ZDB-FIG-131015-58
Wei et al., 2013 - miR-153 Regulates SNAP-25, Synaptic Transmission, and Neuronal Development. PLoS One   8(2):e57080 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Fig. S6

miR-153 regulates secondary motor neuron development. (A) Immunofluorescence was performed on whole mount zebrafish embryos at 55 hpf using Zn-8 antibodies to label secondary motor neurons. Confocal images were acquired from the same somites for all embryos, as indicated. (B) miR-153 knockdown (miR-153MO) and snap-25a,b overexpression significantly increased the growth of secondary motor neuron axons (arrows). Overexpression of miR-153 or knockdown of snap-25a,b (snap-25a,bMO) caused severe defects in axon development and architecture (asterisks). Scale bar: 40 μm.

Gene Expression DetailsNo data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
zn-8 IHC WT standard conditions Long-pec spinal cord secondary motor neuron
IHC WT + MO1-mir153 + MO1-mir153a standard conditions Long-pec spinal cord secondary motor neuron
IHC WT + MO1-mir153 + MO1-mir153a + MO1-snap25 + MO1-snap25a standard conditions Long-pec spinal cord secondary motor neuron
IHC WT + MO1-snap25 + MO1-snap25a standard conditions Long-pec spinal cord secondary motor neuron
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-mir153 + MO1-mir153a standard conditions Long-pec secondary motor neuron axon increased length, abnormal
Long-pec secondary motor neuron axon extension increased process quality, abnormal
WT + MO1-snap25 + MO1-snap25a standard conditions Long-pec secondary motor neuron axon decreased length, abnormal
Long-pec secondary motor neuron axon extension decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One