FIGURE

Fig. 3

ID

ZDB-FIG-131015-47

Publication

Wei et al., 2013 - miR-153 Regulates SNAP-25, Synaptic Transmission, and Neuronal Development

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Fig. 3

miR-153 regulates endogenous snap-25a expression.

(A) Embryo lysates were prepared from either NIC embryos or embryos injected with miR-153, miR-153^MO, mRNAs encoding snap-25a and snap-25b, morpholinos against snap-25, or combinations thereof, as indicated. Western blots were performed using antibodies against SNAP-25 and α–tubulin. (B) Quantification of SNAP-25 levels from the western blots (n = 3) shown in A. Significance was determined by a two-tailed Student’s t-test. Error bars show s.e.m.

Expression Data

Antibody:	Ab2-snap25
Fish:	WT WT + MO1-dre-mir-153a + MO1-mir153 WT + MO1-snap25 + MO1-snap25a WT + MO1-dre-mir-153a + MO1-mir153 + MO1-snap25 + MO1-snap25a
Knockdown Reagents:	MO1-dre-mir-153a MO1-mir153 MO1-snap25 MO1-snap25a
Anatomical Term:	whole organism
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-dre-mir-153a + MO1-mir153 WT + MO1-snap25 + MO1-snap25a
Knockdown Reagents:	MO1-dre-mir-153a MO1-mir153 MO1-snap25 MO1-snap25a
Observed In:	whole organism SNARE complex
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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