FIGURE

Fig. S3

ID

ZDB-FIG-131015-55

Publication

Wei et al., 2013 - miR-153 Regulates SNAP-25, Synaptic Transmission, and Neuronal Development

Other Figures

All Figure Page

Back to All Figure Page

Fig. S3

miR-153 targets snap-25b. (A) GFP reporter constructs were created by fusing the reading frame of GFP to the snap-25b 32UTR. Three predicted miRNA recognition elements (MREs) were identified in the snap-25b 32 UTR. The miR-153 sequence is indicated in red and the corresponding snap-25a UTR sequence is shown in green. (B) Single cell zebrafish embryos were injected with mRNAs derived from GFP reporters lacking a UTR (GFP), fused to the full length snap-25b UTR (GFP+snap-25b), or mutant version of the snap-25b UTR lacking all MREs (GFP+snap-25bΔMRE1, 2&3). Embryos were injected in the presence or absence of exogenous miR-153 or morpholinos against miR-153 (miR-153^MO). Fluorescence levels were examined at 1 dpf. Clusters of embryos (~30) are shown. (C)Lysates from ~100 embryos were prepared from embryos treated as in B and GFP protein levels were determined by western blotting using antibodies against GFP or control antibodies against α-tubulin.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One