FIGURE

Fig. 6

ID

ZDB-FIG-130816-1

Publication

Nishiwaki et al., 2013 - The BH3-Only SNARE BNip1 Mediates Photoreceptor Apoptosis in Response to Vesicular Fusion Defects

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Fig. 6

The N-Terminal Coiled-Coil Domain Suppresses BNip1 Proapoptotic Activity(A) BNip1b has four distinct domains: a coiled-coil domain, a BH3 domain, a SNARE domain, and a TM domain. Configuration of deletion constructs (BNip1b-ΔTM, BNip1b-ΔSNARE, BNip1b-Δcc, BNip1b-Δcc-BH3(L114A), and BNip1b-Δcc-ΔBH3). (B) Activation of caspase 3 at 7 hpf (upper rows) and morphologies at 8 hpf (lower rows) in embryos injected with mRNA encoding BNip1b, BNip1b-ΔTM, BNip1b-ΔSNARE, BNip1b-Δcc, and BNip1b-Δcc-ΔBH3. Severe embryonic death and morphological defects are observed only in BNip1Δcc-expressing embryos. (C) Percentages of 8 hpf embryos classified as classes I–IV in the injection experiments shown in (B) (lower panels), as well as BNip1b-Δcc-BH3(L114A). (D) Percentage of class I/II embryos. Green and black bars indicate the means and SDs, respectively. The numbers of injection experiments and t test p values are shown in Table S2. ^**p < 0.01. Scale bars, 200 μm (B, upper panels) and 600 μm (B, lower panels). See also Figure S7.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 25(4), Nishiwaki, Y., Yoshizawa, A., Kojima, Y., Oguri, E., Nakamura, S., Suzuki, S., Yuasa-Kawada, J., Kinoshita-Kawada, M., Mochizuki, T., and Masai, I., The BH3-Only SNARE BNip1 Mediates Photoreceptor Apoptosis in Response to Vesicular Fusion Defects, 374-387, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell