PUBLICATION

The BH3-Only SNARE BNip1 Mediates Photoreceptor Apoptosis in Response to Vesicular Fusion Defects

Authors
Nishiwaki, Y., Yoshizawa, A., Kojima, Y., Oguri, E., Nakamura, S., Suzuki, S., Yuasa-Kawada, J., Kinoshita-Kawada, M., Mochizuki, T., and Masai, I.
ID
ZDB-PUB-130708-49
Date
2013
Source
Developmental Cell   25(4): 374-387 (Journal)
Registered Authors
Masai, Ichiro, Mochizuki, Toshiaki, Nishiwaki, Yuko
Keywords
none
MeSH Terms
  • Qa-SNARE Proteins/genetics
  • Qa-SNARE Proteins/metabolism
  • Protein Structure, Tertiary
  • Endoplasmic Reticulum/metabolism
  • Multiprotein Complexes/genetics
  • Multiprotein Complexes/metabolism
  • Retinal Neurons/metabolism
  • Retinal Neurons/pathology
  • Golgi Apparatus/metabolism
  • Apoptosis*
  • Mutation
  • Proto-Oncogene Proteins c-bcl-2/genetics
  • Proto-Oncogene Proteins c-bcl-2/metabolism*
  • Protein Transport
  • Protein Interaction Mapping
  • Animals
  • Retinal Cone Photoreceptor Cells/metabolism
  • Retinal Cone Photoreceptor Cells/pathology*
  • Zebrafish/genetics
  • Zebrafish/metabolism
  • Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins/genetics
  • Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins/metabolism*
  • Membrane Fusion*
(all 23)
PubMed
23725763 Full text @ Dev. Cell
Abstract

Intracellular vesicular transport is important for photoreceptor function and maintenance. However, the mechanism underlying photoreceptor degeneration in response to vesicular transport defects is unknown. Here, we report that photoreceptors undergo apoptosis in a zebrafish β-soluble N-ethylmaleimide-sensitive factor attachment protein (β-SNAP) mutant. β-SNAP cooperates with N-ethylmaleimide-sensitive factor to recycle the SNAP receptor (SNARE), a key component of the membrane fusion machinery, by disassembling the cis-SNARE complex generated in the vesicular fusion process. We found that photoreceptor apoptosis in the β-SNAP mutant was dependent on the BH3-only protein BNip1. BNip1 functions as a component of the syntaxin-18 SNARE complex and regulates retrograde transport from the Golgi to the endoplasmic reticulum. Failure to disassemble the syntaxin-18 cis-SNARE complex caused BNip1-dependent apoptosis. These data suggest that the syntaxin-18 cis-SNARE complex functions as an alarm factor that monitors vesicular fusion competence and that BNip1 transforms vesicular fusion defects into photoreceptor apoptosis.

Genes / Markers
Figures
Figure Gallery (12 images) / 2
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
hi2869TgTransgenic Insertion
oki04TgTransgenic Insertion
    rw021TgTransgenic Insertion
      rw76b
        Point Mutation
        st53
          Point Mutation
          1 - 5 of 5
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          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          Target Reagent Reagent Type
          baxaMO2-baxaMRPHLNO
          baxbMO1-baxbMRPHLNO
          bnip1aMO1-bnip1aMRPHLNO
          bnip1bMO1-bnip1bMRPHLNO
          nsfaMO1-nsfaMRPHLNO
          scfd1MO1-scfd1MRPHLNO
          sec22baMO1-sec22baMRPHLNO
          stx5alMO1-stx5alMRPHLNO
          stx18MO1-stx18MRPHLNO
          use1MO1-use1MRPHLNO
          1 - 10 of 10
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          Fish
          Antibodies
          Orthology
          No data available
          Engineered Foreign Genes
          Marker Marker Type Name
          GFPEFGGFP
          1 - 1 of 1
          Show
          Mapping
          Entity Type Entity Symbol Location
          Featurerw76bChr: 20 Details
          GENEgpcpd1Chr: 20 Details
          GENEtrib2Chr: 20 Details
          SSLPz59759Chr: 20 Details
          1 - 4 of 4
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