FIGURE

Fig. 5

ID

ZDB-FIG-130709-10

Publication

Chandrasekar et al., 2013 - The Zebrafish Orthologue of the Dyslexia Candidate Gene DYX1C1 Is Essential for Cilia Growth and Function

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Fig. 5

Knockdown of dyx1c1 reduced cilia length and number in different organs

Immunolabelling of KV cilia with anti-acetylated tubulin in WT (A) and dyx1c1 morphants (B) at 14 hpf showed reduction in cilia length in morphant embryos. Compared to WT control (C), morphant embryo (D) revealed fewer and shortened cilia in the olfactory placode. At 2 dpf, cilia in dyx1c1 morphants were shortened in pronephros and spinal canal (F, H) compared to WT (E, G). Measurement of cilia number in WT and morphants (I). Confocal images of KV in WT and morphant embryos at 14 hpf stained with anti-acetylated tubulin and DAPI (J, K). Graphical representation of total number of nuclei/KV in WT and morphant embryos (L). Panel C, D, E, F, G and H are lateral views with anterior to the left in E, F, G and H. Scale bars indicate 10 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-dnaaf4 + MO2-dnaaf4
Knockdown Reagents:	MO1-dnaaf4 MO2-dnaaf4
Observed In:	Kupffer's vesicle cilium peripheral olfactory organ cilium pronephros cilium spinal cord cilium
Stage Range:	10-13 somites to Long-pec

Phenotype Detail

Acknowledgments

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