FIGURE

Fig. 6

ID

ZDB-FIG-130627-42

Publication

Stuckenholz et al., 2013 - Sfrp5 Modulates Both Wnt and BMP Signaling and Regulates Gastrointestinal Organogensis in the Zebrafish, Danio rerio

Other Figures

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Fig. 6

Endodermal defects in embryos with altered levels of Sfrp5.

Embryos were injected with 0.5 pmol morpholino against sfrp5 (MO; panels B, G, L), 0.5 pmol of the control morpholino (mmMO; panels A, F, K), 100 pg mCherry mRNA (C, H, M), or 100 pg sfrp5 mRNA (D, E, I, J, N, O). A–O) Whole-mount in situ hybridization with a probe against foxa1 staining embryos at 25 hpf (A–E), 36 hpf (F–J), and 48 hpf (K–O). Dorsal view, anterior to the left. Arrowheads point to the hepatoblast (F–J). P) Bar chart representing distribution of normal and abnormal embryos processed by in situ hybridization, with representative samples shown in A–O. Below each column, numbers of embryos analyzed.

Expression Data

Gene:	foxa1
Fish:	WT WT + MO1-sfrp5
Knockdown Reagent:	MO1-sfrp5
Anatomical Term:	endoderm
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-sfrp5
Knockdown Reagent:	MO1-sfrp5
Observed In:	endoderm
Stage Range:	Prim-5 to Long-pec

Phenotype Detail

Acknowledgments

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