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Fig. 6 Endodermal defects in embryos with altered levels of Sfrp5.
Embryos were injected with 0.5 pmol morpholino against sfrp5 (MO; panels B, G, L), 0.5 pmol of the control morpholino (mmMO; panels A, F, K), 100 pg mCherry mRNA (C, H, M), or 100 pg sfrp5 mRNA (D, E, I, J, N, O). A–O) Whole-mount in situ hybridization with a probe against foxa1 staining embryos at 25 hpf (A–E), 36 hpf (F–J), and 48 hpf (K–O). Dorsal view, anterior to the left. Arrowheads point to the hepatoblast (F–J). P) Bar chart representing distribution of normal and abnormal embryos processed by in situ hybridization, with representative samples shown in A–O. Below each column, numbers of embryos analyzed.