Fig. 3
sfrp5 overexpression inhibits non-canonical Wnt signaling. Embryos injected with 200 pg mCherry, 140 pg sfrp5, and 100 pg wnt11b from Xenopus laevis alone and in combination as indicated. A–D) 4-somite embryos were processed by in situ hybridization with probes against ctsl1b, dlx3b, and ntla. Dorsal view with anterior towards the top. The neural plate was scored as normal (A), widened (B), or widened with a laterally (C) or centrally (D) displaced prechordal plate. E) Bar chart showing percentage of different phenotypic classes for each treatment. The total number of embryos analyzed per treatment is shown under each column. |