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ZFIN ID: ZDB-FIG-130515-11
Riley et al., 2010 - Characterization of harpy/Rca1/emi1 mutants: patterning in the absence of cell division. Developmental dynamics : an official publication of the American Association of Anatomists   239(3):828-843 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Prim-15
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-5 to Prim-15

Fig. 5

Neural patterning defects in harpy mutants. Panels show wild-type control embryos and harpy mutants. A,B: Expression of deltaA (dlA) at 24 hr. Dorsal view of hindbrain. Note the normal intensity of expression in mutant cells. C,D: Expression of notch1b at 24 hr. Dorsal view of head; insets show lateral view. Note expression in mutants is almost absent. E,F: Expression of fgf8 at 24 hr. Dorsal view of hindbrain. Note ectopic staining in the fourth rhombomere of the mutant. G,H: Anti-Pax7 staining at 24 hr. Arrows indicate neural crest cells migrating over the eye and pharyngeal arches; while these cells are present in the mutant, they are not seen on the migration pathway. I,J: Dorsal view of 3A10-stained Mauthner neurons at 30 hr, displaying abnormal pathfinding and absence of axons in the mutants. K,L: Anti-Grasp staining at 30 hr; dorsal view of the hindbrain. Arrows indicate commissural neurons that in the mutant are chaotic. M,N: Anti-acetylated tubulin (AT) staining at 24 hr; dorsal view of the trunk. Although axons are present in mutants, they do not express acetylated tubulin except in the tail region. mhb, midbrain–hindbrain border; mth, Mauthner neurons; ov, otic vesicle; r4, fourth rhombomere. Scale bar = 50 μm in A–D, 20 μm in F,G, 100 μm in E–N.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dla WT standard conditions Prim-5 proliferative region neuroblast (sensu Vertebrata) ISH
fbxo5x1/x1 standard conditions Prim-5 proliferative region neuroblast (sensu Vertebrata) ISH
fgf8a WT standard conditions Prim-5 midbrain hindbrain boundary ISH
Prim-5 otic vesicle ISH
fbxo5x1/x1 standard conditions Prim-5 midbrain hindbrain boundary ISH
Prim-5 otic vesicle ISH
Prim-5 rhombomere 4 ISH
notch1b WT standard conditions Prim-5 central nervous system neuronal stem cell ISH
fbxo5x1/x1 standard conditions Prim-5 central nervous system neuronal stem cell ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-3A10 IHC WT standard conditions Prim-15 Mauthner neuron
IHC fbxo5x1/x1 standard conditions Prim-15 Mauthner neuron
Ab-Pax7 IHC WT standard conditions Prim-15 neural crest cell
IHC Prim-15 trunk neural crest
IHC fbxo5x1/x1 standard conditions Prim-15 neural crest cell
zn-8 IHC WT standard conditions Prim-15 hindbrain commissure
IHC fbxo5x1/x1 standard conditions Prim-15 hindbrain commissure
Phenotype Details
Fish Conditions Stage Phenotype
fbxo5x1/x1 standard conditions Prim-5 hindbrain dorsal region disorganized, abnormal
Prim-5 neural crest cell migration disrupted, abnormal
Prim-5 neuron microtubule molecular quality, abnormal
Prim-5 rhombomere 4 morphogenesis disrupted, abnormal
Prim-15 commissural neuron axon guidance disrupted, abnormal
Prim-15 hindbrain commissure axon disorganized, abnormal
Prim-15 interneuron axon guidance disrupted, abnormal
Prim-15 Mauthner neuron axon decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.