Fig. 8
The GJ inhibitor CBX inhibits breast cancer brain colonization. (A,B) Chickens were treated with (A) vehicle or (B) CBX (2.5mg/egg/day) starting 1day before tumor cell injection. Microtumor formation in the brain was assessed at the indicated days using confocal microscopy as described for Fig.6B. Insets show higher magnification (60×) of the areas outlined by the dashed squares. The brain vasculature is labeled with Rhodamine–lectin dye (red). (C) Average length of tumor-cell blood vessel contacts for 4T-1 cells from the brains of vehicle or CBX-treated chicken 6 days after injection. (D) Number and volume of microtumors per chicken brain induced by 4T-1 tumor cells for vehicle or CBX-treated chickens 6 days after injection. Scale bars: 200 μm. |