FIGURE

Fig. 3

ID

ZDB-FIG-130510-37

Publication

Stoletov et al., 2013 - Role of connexins in metastatic breast cancer and melanoma brain colonization

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Fig. 3

Metastatic breast cancer cell extravasation and brain colonization require Cx43 expression. (A) 4T-1 control or 4T-1KNCx43 cells expressing dsRed were injected into the circulation of Tg(fli1:egfp) transgenic zebrafish and allowed to extravasate in the brain for 24hours. Shown are representative 3D confocal reconstructions of the brain vasculature (green) and tumor cells (red) in the head region. (B) Average percentage of 4T-1 control and 4T-1KNcx43 cells that extravasated out of the vasculature into the brain parenchyma 24hours after injection. (C) Average percentage of extravasated 4T-1 control and 4T-1KNCx43 cells with an elongated invasive morphology in the brain parenchyma. Scale bar: 200μm. (D) 4T-1 control or 4T-1KNCx43 cells expressing GFP were injected into the chicken circulation and allowed to form microtumors in the brain for 7 days. Shown are representative 3D confocal reconstructions of the brain vasculature (red, Rhodamine–lectin stained) and tumor cells (green) (60×). Insets show lower magnification (10×); dashed squares indicate areas enlarged in the main panels. Note that 3D rendering shows that 4T-1 control cells co-opt brain blood vessels whereas 4T-1KNCx43 cells show little vessel association. (E) Number and volume of 4T-1 control and 4T-1KNCx43 microtumor lesions in the chicken brain after 7 days. (F) Average length of tumor-cell blood vessel contacts for 4T-1 control and 4T-1KNCx43 tumor cells after 7 days in the chicken brain. Scale bars: 20μm (main panels), 200μm (insets).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.