FIGURE

Fig. 2

ID

ZDB-FIG-130426-16

Publication

Choe et al., 2013 - Wnt-Dependent Epithelial Transitions Drive Pharyngeal Pouch Formation

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Fig. 2

Expression of wnt11r, wnt4a, and fzd8a during Pouch Formation(A–F) Colorimetric in situs show expression of wnt11r in discrete domains of mesoderm (arrowheads in A and B), wnt4a in ectodermal patches (arrowheads in C and D), and fzd8a in pouch-forming endoderm (E and F).(G–J) Double fluorescent in situs show colocalization of wnt11r but not wnt4a with nkx2.5 in lateral views (G and I) and higher magnification orthogonal sections (H and J, taken at level of white lines in G and I). Arrowheads indicate mesoderm and arrows ectoderm.(K) Fluorescent in situ shows colocalization of fzd8a (green) with her5-positive pouch endoderm labeled by GFP immunohistochemistry (red).(L) Schematic showing expression of wnt11r in mesoderm (blue), wnt4a in ectoderm (yellow), and fzd8a in endoderm (red) during pouch formation.(M–P) Compared to un-injected siblings (M and O), injection of 5 nl of 5 mM Mtz into nkx2.5:Gal4VP16; UAS:CFP-NTR embryos results in reduced numbers of mesodermal cells expressing nkx2.5 and wnt11r (arrowheads), as well as reduced ectodermal wnt4a expression. Scale bars, 20 μM.

Expression Data

Genes:	fzd8a mCherry nkx2.5 wnt4 wnt11
Fish:	WT el72Tg el53Tg; el74Tg
Condition:	chemical treatment: metronidazole
Anatomical Terms:	pharyngeal pouch ectodermal cell pharyngeal pouch endodermal cell pharyngeal pouch mesodermal cell
Stage Range:	Prim-5 to Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	el53Tg; el74Tg
Condition:	chemical treatment: metronidazole
Observed In:	pharyngeal pouch pharyngeal pouch ectodermal cell pharyngeal pouch mesodermal cell
Stage:	Prim-15

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 24(3), Choe, C.P., Collazo, A., Trinh, L.A., Pan, L., Moens, C.B., and Crump, J.G., Wnt-Dependent Epithelial Transitions Drive Pharyngeal Pouch Formation, 296-309, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell