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ZFIN ID: ZDB-FIG-130426-17
Choe et al., 2013 - Wnt-Dependent Epithelial Transitions Drive Pharyngeal Pouch Formation. Developmental Cell   24(3):296-309 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Prim-15
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-15 to Day 5

Fig. 3 Roles of Wnt Signaling Components in Pouch and CB Cartilage Development(A–L) Alcama immunohistochemistry (green) shows defects in pouches in mutant, fzd8a-MO, and transgenic embryos. Identifiable pouches are numbered. UAS transgenic embryos (capitalized) were doubly positive for nkx2.3:Gal4VP16. Scale bar, 40 μM.(M–X) Whole-mount views of dissected facial cartilages. Arrows indicated fused or abnormal CB cartilages. Arrowhead indicates abnormal hyoid cartilage in fzd8a-MO embryos.(Y–AA) Superimposition of initial (blue) and final (red) still images from time-lapse recordings of fifth pouch development in wild-type, wnt11r-/-, and wnt4a-/- embryos. Colored lines indicate cell tracks (shown also below merged images), with filled circles denoting final positions.(AB) Average pouch cell speed in wild-types and mutants. Data represent mean ± SEM and p values are shown for each comparison.See also Figures S1 and S2 and Movies S3, S4, and S5.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
alcama zn-8 WT standard conditions Prim-15 pharyngeal pouch cell-cell junction IHC
zn-8 WT + MO2-fzd8a standard conditions Prim-15 pharyngeal pouch cell-cell junction IHC
zn-8 el190Tg; el93Tg standard conditions Prim-15 pharyngeal pouch cell-cell junction IHC
zn-8 el262Tg; el93Tg standard conditions Prim-15 pharyngeal pouch cell-cell junction IHC
zn-8 el268Tg; el93Tg standard conditions Prim-15 pharyngeal pouch cell-cell junction IHC
zn-8 el320Tg; el93Tg standard conditions Prim-15 pharyngeal pouch cell-cell junction IHC
zn-8 el322Tg; el93Tg standard conditions Prim-15 pharyngeal pouch cell-cell junction IHC
zn-8 el421Tg; el93Tg standard conditions Prim-15 pharyngeal pouch cell-cell junction IHC
zn-8 el48Tg; el93Tg standard conditions Prim-15 pharyngeal pouch cell-cell junction IHC
zn-8 wnt11rfh224/fh224 standard conditions Prim-15 pharyngeal pouch cell-cell junction IHC
zn-8 wnt11rfh224/fh224; wnt4fh295/fh295 standard conditions Prim-15 pharyngeal pouch cell-cell junction IHC
zn-8 wnt4afh295/fh295 standard conditions Prim-15 pharyngeal pouch cell-cell junction IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-fzd8a standard conditions Prim-15 pharyngeal pouch malformed, abnormal
Day 5 ceratobranchial cartilage malformed, abnormal
Day 5 embryonic viscerocranium morphogenesis decreased process quality, abnormal
el190Tg; el93Tg standard conditions Prim-15 pharyngeal pouch malformed, abnormal
Prim-15 whole organism has fewer parts of type pharyngeal pouch, abnormal
Day 5 ceratobranchial cartilage malformed, abnormal
Day 5 embryonic viscerocranium morphogenesis decreased process quality, abnormal
el262Tg; el93Tg standard conditions Prim-15 pharyngeal pouch malformed, abnormal
Prim-15 whole organism has fewer parts of type pharyngeal pouch, abnormal
Day 5 ceratobranchial cartilage malformed, abnormal
Day 5 embryonic viscerocranium morphogenesis decreased process quality, abnormal
el268Tg; el93Tg standard conditions Prim-15 pharyngeal pouch malformed, abnormal
Prim-15 whole organism has fewer parts of type pharyngeal pouch, abnormal
Day 5 ceratobranchial cartilage malformed, abnormal
Day 5 embryonic viscerocranium morphogenesis decreased process quality, abnormal
el320Tg; el93Tg standard conditions Prim-15 pharyngeal pouch malformed, abnormal
Prim-15 whole organism has fewer parts of type pharyngeal pouch, abnormal
Day 5 ceratobranchial cartilage malformed, abnormal
Day 5 embryonic viscerocranium morphogenesis decreased process quality, abnormal
el322Tg; el93Tg standard conditions Prim-15 pharyngeal pouch malformed, abnormal
Prim-15 whole organism has fewer parts of type pharyngeal pouch, abnormal
Day 5 ceratobranchial cartilage malformed, abnormal
Day 5 embryonic viscerocranium morphogenesis decreased process quality, abnormal
el421Tg; el93Tg standard conditions Prim-15 pharyngeal pouch malformed, abnormal
Prim-15 whole organism has fewer parts of type pharyngeal pouch, abnormal
Day 5 ceratobranchial cartilage malformed, abnormal
Day 5 embryonic viscerocranium morphogenesis decreased process quality, abnormal
el48Tg; el93Tg standard conditions Prim-15 pharyngeal pouch malformed, abnormal
Prim-15 whole organism has fewer parts of type pharyngeal pouch, abnormal
Day 5 ceratobranchial cartilage malformed, abnormal
Day 5 embryonic viscerocranium morphogenesis decreased process quality, abnormal
wnt11rfh224/fh224 standard conditions Prim-15 pharyngeal pouch malformed, abnormal
Prim-15 pharyngeal pouch cell migration decreased speed, abnormal
Prim-15 whole organism has fewer parts of type pharyngeal pouch, abnormal
Day 5 ceratobranchial cartilage malformed, abnormal
Day 5 embryonic viscerocranium morphogenesis decreased process quality, abnormal
wnt11rfh224/fh224; wnt4fh295/fh295 standard conditions Prim-15 pharyngeal pouch malformed, abnormal
Prim-15 whole organism has fewer parts of type pharyngeal pouch, abnormal
Day 5 ceratobranchial cartilage malformed, abnormal
Day 5 embryonic viscerocranium morphogenesis decreased process quality, abnormal
wnt4afh295/fh295 standard conditions Prim-15 pharyngeal pouch malformed, abnormal
Prim-15 pharyngeal pouch cell migration increased speed, abnormal
Prim-15 whole organism has normal numbers of parts of type pharyngeal pouch, normal
Day 5 ceratobranchial cartilage malformed, abnormal
Day 5 embryonic viscerocranium morphogenesis decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Cell, 24(3), Choe, C.P., Collazo, A., Trinh, L.A., Pan, L., Moens, C.B., and Crump, J.G., Wnt-Dependent Epithelial Transitions Drive Pharyngeal Pouch Formation, 296-309, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell