FIGURE

Fig. 3

ID

ZDB-FIG-130426-17

Publication

Choe et al., 2013 - Wnt-Dependent Epithelial Transitions Drive Pharyngeal Pouch Formation

Other Figures

(all 9)

All Figure Page

Back to All Figure Page

Fig. 3

Roles of Wnt Signaling Components in Pouch and CB Cartilage Development(A?L) Alcama immunohistochemistry (green) shows defects in pouches in mutant, fzd8a-MO, and transgenic embryos. Identifiable pouches are numbered. UAS transgenic embryos (capitalized) were doubly positive for nkx2.3:Gal4VP16. Scale bar, 40 μM.(M?X) Whole-mount views of dissected facial cartilages. Arrows indicated fused or abnormal CB cartilages. Arrowhead indicates abnormal hyoid cartilage in fzd8a-MO embryos.(Y?AA) Superimposition of initial (blue) and final (red) still images from time-lapse recordings of fifth pouch development in wild-type, wnt11r^-/-, and wnt4a^-/- embryos. Colored lines indicate cell tracks (shown also below merged images), with filled circles denoting final positions.(AB) Average pouch cell speed in wild-types and mutants. Data represent mean ± SEM and p values are shown for each comparison.See also Figures S1 and S2 and Movies S3, S4, and S5.

Expression Data

Gene:	alcama
Antibody:	zn-8
Fish:	WT wnt4^fh295/fh295 wnt11^fh224/fh224 WT + MO2-fzd8a el48Tg; el93Tg el190Tg; el93Tg el262Tg; el93Tg el268Tg; el93Tg el320Tg; el93Tg el322Tg; el93Tg el421Tg; el93Tg wnt11^fh224/fh224; wnt4^fh295/fh295 (all 12)
Knockdown Reagent:	MO2-fzd8a
Anatomical Term:	pharyngeal pouch cell-cell junction
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	wnt4^fh295/fh295 wnt11^fh224/fh224 WT + MO2-fzd8a el48Tg; el93Tg el190Tg; el93Tg el262Tg; el93Tg el268Tg; el93Tg el320Tg; el93Tg el322Tg; el93Tg el421Tg; el93Tg wnt11^fh224/fh224; wnt4^fh295/fh295 (all 11)
Knockdown Reagent:	MO2-fzd8a
Observed In:	ceratobranchial cartilage embryonic viscerocranium morphogenesis pharyngeal pouch pharyngeal pouch cell migration whole organism
Stage Range:	Prim-15 to Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Cell, 24(3), Choe, C.P., Collazo, A., Trinh, L.A., Pan, L., Moens, C.B., and Crump, J.G., Wnt-Dependent Epithelial Transitions Drive Pharyngeal Pouch Formation, 296-309, Copyright (2013) with permission from Elsevier. Full text @ Dev. Cell