FIGURE

Fig. 3

ID

ZDB-FIG-130404-7

Publication

Law et al., 2013 - Maternal pak4 Expression is Required for Primitive Myelopoiesis in Zebrafish

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Fig. 3

pak4 is required for primitive myelopoiesis. (A–C) Inhibition of gata2a expression by MZpak4 knockdown in the posterior blood island (red arrows) in 24-hpf embryos. (D–I) Inhibited expression of the hematopoietic regulatory factor scl in the rostral blood island (RBI; red arrows) at 5-somite stage, lateral view (D–F) and 7-somite stage flat mounts (G–I). (A, D and G) Control morphants, (B, E and H) MZpak4 knockdown embryos. (C, F and I) MZpak4 knockdown embryos rescued by pak4 mRNA injection. (J) Relative expression levels of scl in anterior and posterior regions at 5-somite stage, l-plastin at 15-somite and mpo at 20-somite stages. mRNA levels were measured by QPCR, normalized to 18S rRNA levels and represented as a ratio to the corresponding uninjected control embryos. Samples for anterior and posterior measurement of scl were dissected across the axis as shown while those for l-plastin and mpo were from whole embryos. Results shown are representatives from two individual experiments with similar results.

Expression Data

Genes:	gata2a lcp1 mpx tal1
Fish:	EKW EKW + MO3-pak4 + MO4-pak4 + MO5-pak4
Knockdown Reagents:	MO3-pak4 MO4-pak4 MO5-pak4
Anatomical Terms:	blood island rostral blood island whole organism
Stage Range:	5-9 somites to Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	EKW + MO3-pak4 + MO4-pak4 + MO5-pak4
Knockdown Reagents:	MO3-pak4 MO4-pak4 MO5-pak4
Observed In:	myeloid cell differentiation
Stage Range:	5-9 somites to 20-25 somites

Phenotype Detail

Acknowledgments

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Reprinted from Mechanisms of Development, 130(2-3), Law, S.H., and Sargent, T.D., Maternal pak4 Expression is Required for Primitive Myelopoiesis in Zebrafish, 181-194, Copyright (2013) with permission from Elsevier. Full text @ Mech. Dev.