FIGURE

Fig. S1

ID

ZDB-FIG-130401-4

Publication

Tovin et al., 2012 - Systematic Identification of Rhythmic Genes Reveals camk1gb as a New Element in the Circadian Clockwork

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Fig. S1

Whole-mount ISH and qRT-PCR validations. The circadian expression of several genes was validated using whole mount ISH on embryos at the age of 48–72 hours (A–E, right curves) and qRT-PCR on adult pineal glands (F–H, right curves). Left curves represent the circadian profile of each gene as obtained by RNA-seq (solid line, left vertical bar) and DNA-microarray (dashed line, right vertical bar). Representative pictures of embryos heads (dorsal view), subjected to whole mount ISH for ndrg1b, dec1, dec2, reverbb2 and sh3gl2 at CT2 and CT14, are presented at the rightmost side of A–E. The pineal gland is indicated by a red arrow. Different letters represent statistical differences in mRNA levels as determined by one-way ANOVA followed by a Tukey test (P-value<0.05). Note that cdh2 was not validated as circadian (H). Error bars represent SE (n = 10–15). CT = circadian time. Gray and black bars represent subjective day and subjective night, respectively. Whole mount ISH validation of camk1gb is given in Figure 3.

Expression Data

Genes:	bhlhe40 bhlhe41 ndrg1b nr1d2b sh3gl2a
Fish:	WT
Anatomical Term:	epiphysis
Stage Range:	Long-pec to Pec-fin

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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