FIGURE

Fig. 5

ID

ZDB-FIG-130401-3

Publication

Tovin et al., 2012 - Systematic Identification of Rhythmic Genes Reveals camk1gb as a New Element in the Circadian Clockwork

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Fig. 5

Effect of camk1gb knockdown on pineal aanat2 and otx5 mRNA rhythms.Zebrafish embryos injected with either control morpholino (black line) or camk1gb morpholino (gray line) were subjected to DD during their third day of development and pineal aanat2 and otx5 mRNA levels were determined by whole mount ISH. A) Embryos were sampled at 4-hr intervals for aanat2. Statistical differences in aanat2 mRNA levels between the control morpholino and camk1gb morpholino injected embryos were determined by two-tailed t-test with Bonferroni correction (* P-value<0.05, ** P-value<0.01, *** P-value<0.001). B) Whole-mount ISH for aanat2 in the heads (dorsal views) of representative larvae from each group, at CT14 and CT18. Arrows indicate pineal aanat2 mRNA expression. C) otx5 expression at CT18. Error bars represent SE (n = 10–15). CT = circadian time.

Expression Data

Genes:	aanat2 otx5
Fish:	WT WT + MO1-camk1gb
Knockdown Reagent:	MO1-camk1gb
Anatomical Term:	epiphysis
Stage:	Pec-fin

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-camk1gb
Knockdown Reagent:	MO1-camk1gb
Observed In:	epiphysis circadian regulation of gene expression pineal gland development
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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