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ZFIN ID: ZDB-FIG-130401-16
Seredick et al., 2012 - Zebrafish Mnx proteins specify one motoneuron subtype and suppress acquisition of interneuron characteristics. Neural Development   7(1):35 Full text @ Neural Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 14-19 somites to 20-25 somites

Fig. 2 Mnx proteins are not required for formation of PMNs or VeLDs. Control and mnx MO-injected embryos labeled with markers of PMN or VeLD identity. (A-B) At 16 hpf, PMNs express chat and nrp1a:GFP in control (A) and mnx MO-injected embryos (B). (C-D) At 18 hpf, PMNs express Islet in control (C) and mnx MO-injected Tg(nrp1a:GFP) embryos (D). (E-F) At 20 hpf, VeLD interneuron (arrowheads) numbers and distribution are the same in control (E) and mnx MO-injected Tg(vsx1:GFP) embryos (F); n = 80 segments in 10 embryos for control and mnx MO-injected samples. Scale bar: 15 μm, A-B; 20 μm, C-F.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
chata js12Tg standard conditions 14-19 somites primary motor neuron IHC
js12Tg + MO1-mnx2a + MO1-mnx2b + MO5-mnx1 standard conditions 14-19 somites primary motor neuron IHC
GFP js12Tg standard conditions 14-19 somites primary motor neuron IHC
js12Tg + MO1-mnx2a + MO1-mnx2b + MO5-mnx1 standard conditions 14-19 somites primary motor neuron IHC
nns5Tg standard conditions 20-25 somites VeLD IHC
nns5Tg + MO1-mnx2a + MO1-mnx2b + MO5-mnx1 standard conditions 20-25 somites VeLD IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-gad IHC nns5Tg standard conditions 20-25 somites VeLD
IHC nns5Tg + MO1-mnx2a + MO1-mnx2b + MO5-mnx1 standard conditions 20-25 somites VeLD
Ab2-isl IHC js12Tg standard conditions 14-19 somites primary motor neuron
IHC js12Tg + MO1-mnx2a + MO1-mnx2b + MO5-mnx1 standard conditions 14-19 somites primary motor neuron
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Neural Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Neural Dev.