Mnx proteins are not required for formation of PMNs or VeLDs. Control and mnx MO-injected embryos labeled with markers of PMN or VeLD identity. (A-B) At 16 hpf, PMNs express chat and nrp1a:GFP in control (A) and mnx MO-injected embryos (B). (C-D) At 18 hpf, PMNs express Islet in control (C) and mnx MO-injected Tg(nrp1a:GFP) embryos (D). (E-F) At 20 hpf, VeLD interneuron (arrowheads) numbers and distribution are the same in control (E) and mnx MO-injected Tg(vsx1:GFP) embryos (F); n = 80 segments in 10 embryos for control and mnx MO-injected samples. Scale bar: 15 μm, A-B; 20 μm, C-F.