ZFIN ID: ZDB-FIG-130322-35
Wanner et al., 2013 - Axon tracts guide zebrafish facial branchiomotor neuron migration through the hindbrain. Development (Cambridge, England)   140(4):906-915 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 14-19 somites to 26+ somites
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: 14-19 somites to 26+ somites

Fig. 6 Pioneer neuron migration is independent of Cdh2-mediated cell adhesion. (A-E) Still images from a representative time-lapse movie of Tg(zCREST1:membRFP/HuC-GFP) embryos injected with Cdh2MO from 18-22 hpf, shown at 1-hour intervals. Arrows indicate the pioneer neurons. (F-J) Analysis of pioneer and follower FBMN behavior before and after the MLF has entered the hindbrain region in uninjected controls. (K-O) Analysis of Cdh2MO time-lapse data comparing pioneer neuron behavior with follower FBMNs before and after the MLF is present. (F,K) Average speed. (G,L) Average posterior velocity. (H,M) Average number of protrusions per neuron per 5-minute time point. (I,N) Average protrusion length per 5-minute time point. (J,O) Average protrusion angle. Error bars indicate s.e.m. *P<0.05, **P<0.01, ***P<0.001. Scale bar: 20 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP ch100Tg; knu3Tg + MO1-cdh2 standard conditions 14-19 somites to 26+ somites branchiomotor neuron IFL
mRFP ch100Tg; knu3Tg + MO1-cdh2 standard conditions 14-19 somites to 26+ somites neuron IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ch100Tg; knu3Tg + MO1-cdh2 standard conditions 14-19 somites - 26+ somites branchiomotor neuron decreased cellular motility, abnormal
14-19 somites - 26+ somites branchiomotor neuron neuron migration decreased velocity, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development