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Fig. 6 Pioneer neuron migration is independent of Cdh2-mediated cell adhesion. (A-E) Still images from a representative time-lapse movie of Tg(zCREST1:membRFP/HuC-GFP) embryos injected with Cdh2MO from 18-22 hpf, shown at 1-hour intervals. Arrows indicate the pioneer neurons. (F-J) Analysis of pioneer and follower FBMN behavior before and after the MLF has entered the hindbrain region in uninjected controls. (K-O) Analysis of Cdh2MO time-lapse data comparing pioneer neuron behavior with follower FBMNs before and after the MLF is present. (F,K) Average speed. (G,L) Average posterior velocity. (H,M) Average number of protrusions per neuron per 5-minute time point. (I,N) Average protrusion length per 5-minute time point. (J,O) Average protrusion angle. Error bars indicate s.e.m. *P<0.05, **P<0.01, ***P<0.001. Scale bar: 20 μm.