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ZFIN ID: ZDB-FIG-130320-29
Choi et al., 2013 - In vivo monitoring of cardiomyocyte proliferation to identify chemical modifiers of heart regeneration. Development (Cambridge, England)   140(3):660-666 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Term:
Stage: Adult

Fig. 3 Hedgehog, Igf and Tgfβ signaling are required for myocardial regeneration in zebrafish. (A) The Shh ligand shha (transgenic reporter) and regulator/response gene ptch2 (transgenic reporter), Igf ligand igf2b (in situ hybridization) and receptor Igfr1 (immunofluorescence), and Tgfβ ligand tgfb3 (in situ hybridization) each showed increased expression in the wound area by 7 dpa. Brackets indicate injury site. Scale bar: 100 μm. (B) Treatment with CyA (10 μM) or NVP AEW541 (2 μM) from 6 to 7 dpa decreased cardiomyocyte proliferation. Mef2, red; PCNA, green. Brackets indicate injury site. Insets: High magnifications of the boxed areas. Arrowheads indicate proliferating cardiomyocytes. Scale bar: 50 μm. (C) Quantification of the effects of CyA (10 μM), NVP AEW541 (2 μM) and SB-431542 (10 μM) on cardiomyocyte proliferation after resection injury. Fish were treated from 6 to 7 dpa. n=9-15, mean±s.e.m. *P<0.01, Student′s t-test. (D) Quantification of the effects of CyA, NVP AEW541 and SB-431542 on proliferation after genetic cardiomyocyte ablation. Fish were treated from 6 to 7 dpa. n=12-13, mean±s.e.m. *P<0.001, Student′s t-test. Data in each graph were obtained from different clutches of fish.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP sb15Tg standard conditions Adult Not Detected cardiac muscle cell IHC
sb15Tg physical alteration: anatomical structure Adult regenerating tissue cardiac muscle cell IHC
umz23Tg standard conditions Adult Not Detected cardiac muscle cell IHC
umz23Tg physical alteration: anatomical structure Adult regenerating tissue cardiac muscle cell IHC
igf2b WT standard conditions Adult Not Detected cardiac muscle cell ISH
WT physical alteration: anatomical structure Adult regenerating tissue cardiac muscle cell ISH
tgfb3 WT standard conditions Adult Not Detected cardiac muscle cell ISH
WT physical alteration: anatomical structure Adult regenerating tissue cardiac muscle cell ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development