ZFIN ID: ZDB-FIG-130320-27
Choi et al., 2013 - In vivo monitoring of cardiomyocyte proliferation to identify chemical modifiers of heart regeneration. Development (Cambridge, England)   140(3):660-666 Full text @ Development
Anatomical Term:
Stage: Day 4

Fig. 1 FUCCI zebrafish for visualizing cardiomyocyte proliferation. (A) Representative maximum intensity projection of the heart of a 4 dpf cmlc2:FUCCI transgenic larva, visualizing non-proliferating (cmlc2:mCherry-zCdt1, red) and proliferating (cmlc2:Venus-hGeminin, green) cardiomyocytes, as depicted in the schematic at the top. Cardiomyocytes with only the Venus-hGeminin signal (arrowheads) were considered to be proliferating. DAPI, blue. Scale bars: 50 μm. (B) Time course of total (red+ and green+) and proliferating (green+) cardiomyocytes from 2 to 6 dpf. Percentages of proliferating cardiomyocytes are indicated above bars. Data are represented as meanĀ±s.e.m. n=13-24 embryos per stage.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
mCherry pd57Tg/+; pd58Tg/+ standard conditions Day 4 cardiac muscle cell IFL
Venus pd57Tg/+; pd58Tg/+ standard conditions Day 4 cardiac muscle cell IFL
Antibody Labeling Details No data available
Phenotype Details No data available
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development