Spatial expression patterns of zebrafish cers in 5 dpf embryos. A: At this stage, cers1 is localized in brain (b), otic vesicle (ov), intestinal bulb (ib), and mid intestine (mi). B:cers2a is expressed ubiquitously, but its expression is pronounced in the brain, otic vesicle, gill arches (ga), intestinal bulb, mid intestine, and myotomes (mt). C:cers2b shows a strong expression in brain, gill arches, intestinal bulb, mid intestine and myotomes. D: At 5dpf, cers3a can be detected in brain, gill arches, liver (li), intestinal bulb, and mid intestine. E:cers3b is expressed prominently in brain, otic vesicle, gill arches, liver, intestinal bulb, and mid intestine. F:cers4a is remarkably expressed in brain, gill arches, pronephros (pn), liver, intestinal bulb, mid intestine, and myotomes. G:cers4b shows an expression which is similar to the one of its paralogue cers4a, localized in brain, gill arches, liver, intestinal bulb, mid intestine, and myotomes. H:cers5 is strongly expressed in brain, gill arches, liver, intestinal bulb, mid intestine, and myotomes. I:cers6 expression is localized in brain, gill arches, pronephros, intestinal bulb, mid intestine, and myotomes. J: A riboprobe specific for the gbx1 gene, expressed in midbrain and hindbrain, was used as a positive control.
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