FIGURE

Fig. 2

ID

ZDB-FIG-130318-35

Publication

Brondolin et al., 2013 - Identification and expression analysis of the zebrafish homologs of the ceramide synthase gene family

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Fig. 2

Spatial expression patterns of zebrafish cers1, cers2a and cers2b in gastrula (8 hours postfertilization [hpf]), 6-somite stage (12 hpf), 18-somite stage (18 hpf), 24 hpf, and 48 hpf embryos. A: cers1 is present in the trigeminal placode (tp), Rohon-Beard sensory neurons (RB), the hematopoietic intermediate cell mass (ICM), brain (b), and pectoral fin (pf). B: At 12 hpf, cers2a is expressed in rhombomere 5 (R5) and the developing notochord (nc), whereas at 18 hpf and 24 hpf, the gene is expressed in paraxial mesoderm (pam) and cloaca (cl), eye (e), including the lens (le), midbrain–hindbrain boundary (mh), and pronephric duct (pd). At 48 hpf, it is found in brain (b), otic vesicle (ov), pectoral fin (pf), myotomes (mt), and pronephric duct (pd). C: cers2b shows an expression in the anterior polster (ap) and paraxial mesoderm (pam), the lens of the eye (le), branchial arches (ba), and posterior somites (ps). The latter is reduced to the posterior mesoderm (pm) at 24 hpf. At 48 hpf, the expression is ubiquitous, brain (b), otic vesicle (ov), pectoral fin (pf), myotomes (mt), and the pronephric duct (pd) indicated by arrows.

Expression Data

Genes:	cers1 cers2a cers2b
Fish:	AB
Anatomical Terms:	brain cloacal chamber eye intermediate cell mass of mesoderm lens placode midbrain hindbrain boundary myotome notochord otic vesicle paraxial mesoderm pectoral fin pharyngeal arch 3-7 polster posterior lateral mesoderm presumptive rhombomere 5 pronephric duct rhombomere 5 Rohon-Beard neuron solid lens vesicle trigeminal ganglion trigeminal neural crest whole organism
Stage Range:	75%-epiboly to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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