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ZFIN ID: ZDB-FIG-130205-18
Huitema et al., 2012 - Entpd5 is essential for skeletal mineralization and regulates phosphate homeostasis in zebrafish. Proceedings of the National Academy of Sciences of the United States of America   109(52):21372-21377 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: Days 7-13
PHENOTYPE:
Fish:
Observed In:
Stage: Days 7-13

Fig. 4 Phosphate homeostasis is disturbed in nob mutants. (A) Nob mutants can mineralize their skeleton in the absence of dgfhu4581 function, and will even show ectopic mineralization (arrows point at protrusions of the cleithrum, a typical feature of dgf mutants). (B) Exogenous phosphate partially rescues the nob mutant phenotype. Arrow points at partially mineralized cleithrum. All images represent lateral views of the head skeleton at 6 dpf. (C) Quantitative PCR on 7-dpf embryos revealed that, compared with siblings (white bars), fgf23 expression levels are significantly down-regulated and npt2a expression is significantly up-regulated in nob mutant embryos (gray bars). Results are expressed as mean ± SEM of three independent experiments, with *P < 0.05 and **P < 0.005.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
entpd5a WT standard conditions Days 7-13 whole organism RTPCR
entpd5ahu3718/hu3718 standard conditions Days 7-13 whole organism RTPCR
fgf23 WT standard conditions Days 7-13 whole organism RTPCR
entpd5ahu3718/hu3718 standard conditions Days 7-13 whole organism RTPCR
phex WT standard conditions Days 7-13 whole organism RTPCR
entpd5ahu3718/hu3718 standard conditions Days 7-13 whole organism RTPCR
phospho1 WT standard conditions Days 7-13 whole organism RTPCR
entpd5ahu3718/hu3718 standard conditions Days 7-13 whole organism RTPCR
slc34a1a WT standard conditions Days 7-13 whole organism RTPCR
entpd5ahu3718/hu3718 standard conditions Days 7-13 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
enpp1hu4581/hu4581; entpd5ahu3718/hu3718 standard conditions Days 7-13 bone tissue mineralized, normal
entpd5ahu3718/hu3718 standard conditions Days 7-13 phosphate ion homeostasis disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA