FIGURE

Fig. 4

ID

ZDB-FIG-130205-18

Publication

Huitema et al., 2012 - Entpd5 is essential for skeletal mineralization and regulates phosphate homeostasis in zebrafish

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Fig. 4

Phosphate homeostasis is disturbed in nob mutants. (A) Nob mutants can mineralize their skeleton in the absence of dgf^hu4581 function, and will even show ectopic mineralization (arrows point at protrusions of the cleithrum, a typical feature of dgf mutants). (B) Exogenous phosphate partially rescues the nob mutant phenotype. Arrow points at partially mineralized cleithrum. All images represent lateral views of the head skeleton at 6 dpf. (C) Quantitative PCR on 7-dpf embryos revealed that, compared with siblings (white bars), fgf23 expression levels are significantly down-regulated and npt2a expression is significantly up-regulated in nob mutant embryos (gray bars). Results are expressed as mean ± SEM of three independent experiments, with *P < 0.05 and **P < 0.005.

Expression Data

Genes:	entpd5a fgf23 phex phospho1 slc34a1a
Fish:	WT entpd5a^{hu3718/hu3718}
Anatomical Term:	whole organism
Stage:	Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	entpd5a^{hu3718/hu3718} enpp1^{hu4581/hu4581}; entpd5a^{hu3718/hu3718}
Observed In:	bone tissue phosphate ion homeostasis
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA