FIGURE

Fig. 3

ID

ZDB-FIG-130123-67

Publication

Bhat et al., 2013 - A gene network that coordinates preplacodal competence and neural crest specification in zebrafish

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Fig. 3

Temporal requirements for tfap2a/c. Expression of neural crest marker foxd3 at 12 hpf (A-E) and 14 hpf (F), and otic marker pax2a at 12 hpf (G-K) and 14 hpf (L) in control embryos (A and G), tfap2a/c morphants (B and H), and tfap2a/c morphants in which hs:tfap2a was activated (HS) at the indicated times (C-F, I-L). Expression domains of pax2a in the otic placode (ot) and pronephros (pn) are indicated, and the midbrain–hindbrain border region is marked with an asterisk. Cranial neural crest was rescued by activating hs:tfap2a at any time between late blastula stage (4 hpf) and 6 somite-stage (12 hpf), whereas rescue of the otic domain of pax2a required activation of the transgene during gastrulation (6-10 hpf). All images show dorsal views with anterior to the top.

Expression Data

Genes:	foxd3 pax2a
Fish:	WT WT + MO2-tfap2c + MO4-tfap2a x24Tg + MO2-tfap2c + MO4-tfap2a
Condition:	heat shock
Knockdown Reagents:	MO2-tfap2c MO4-tfap2a
Anatomical Terms:	midbrain hindbrain boundary neural rod neural crest otic placode pronephric mesoderm pronephros
Stage Range:	5-9 somites to 10-13 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 373(1), Bhat, N., Kwon, H.J., and Riley, B.B., A gene network that coordinates preplacodal competence and neural crest specification in zebrafish, 107-117, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.