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ZFIN ID: ZDB-FIG-121217-46
Goody et al., 2012 - NAD+ biosynthesis ameliorates a zebrafish model of muscular dystrophy. PLoS Biology   10(10):e1001409 Full text @ PLoS Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 14-19 somites to Day 4
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 4 itga6 is upregulated in regenerating muscle and characterization of itga6 MOs.

(A) In situ hybridizations showing itga6 expression (purple). (A1ā€“2) Dorsal view, anterior top. (A3ā€“4, B) Side view, anterior left, dorsal top. (A1-4) Black arrowheads denote somitic expression. itga6 expression is high during early muscle development, then decreases. (B) itga6 is re-expressed in regenerating muscle. itga6, not normally expressed in muscle at 4 dpf, is observed in dystrophic lesions of 4 dpf dag1 morphants (red arrowheads). (C) itga6 MO characterization. Dose response graph. MO1 and MO2 generate the same phenotype and synergize when co-injected. (Dā€“M) Brightfield images, side view, anterior left, dorsal top, 1 dpf embryos. (D-I) Phenotypic analysis of itga6 MOs 1 and 2. Embryos injected with low doses of MO1 (E) or MO2 (F) are morphologically similar to controls (D). Combining the two lower doses of MOs 1 and 2 results in a truncated body axis with myotomes that are narrower in the anterior-posterior dimension (G). The identical phenotype is obtained when higher doses of either MO1 (H) or MO2 (I) are injected. (J-M) Pseudo-genetic epistasis analysis. (J) Siblings, (K) itga6 morphants, (L) wi390-/-/laminin gamma1 mutants, and (M) itga6 MOs;wi390-/-. Note that injection of itga6 MOs into laminin mutants does not change their phenotype, suggesting Itga6 functions in laminin signaling and adhesion. (N) Average MTJ angles of 1 dpf embryos. MTJ angles in morphants, mutants, and morphant/mutants do not significantly differ from one another and are all significantly wider than in sibling controls; **p<0.01; N.S., not significant.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
itga6a WT standard conditions 14-19 somites myotome ISH
14-19 somites myotome regenerating tissue ISH
14-19 somites trunk myotome ISH
Prim-5 post-vent region myotome ISH
WT + MO1-dag1 standard conditions Day 4 myotome regenerating tissue ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-itga6a + MO2-itga6a standard conditions Prim-5 myotome decreased width, abnormal
Prim-5 myotome muscle tendon junction malformed, abnormal
Prim-5 whole organism decreased length, abnormal
lamc1wi390Tg/wi390Tg control Prim-5 myotome muscle tendon junction malformed, abnormal
Prim-5 post-vent region decreased length, abnormal
Prim-5 trunk decreased length, abnormal
lamc1wi390Tg/wi390Tg + MO1-itga6a + MO2-itga6a standard conditions Prim-5 myotome muscle tendon junction malformed, abnormal
Prim-5 post-vent region decreased length, abnormal
Prim-5 trunk decreased length, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Biol.