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ZFIN ID: ZDB-FIG-121214-15
Li et al., 2012 - SUMO1-activating enzyme subunit 1 is essential for the survival of hematopoietic stem/progenitor cells in zebrafish. Development (Cambridge, England)   139(23):4321-4329 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 1-cell to Day 6
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Protruding-mouth to Day 6

Fig. 5 Loss of Sae1 function impairs the sumoylation pathway. (A-D) WISH shows the hbae1 and lyz expression in 23 hpf wild-type fish and ubc9 morphants. Black arrows indicate the ICM. Blue arrows indicate the yolk sac. (E,F) WISH indicates the cmyb expression in 36 hpf wild-type fish and ubc9 morphants. Arrows indicate the AGM of zebrafish. (G-J) WISH shows the cmyb expression in 3-4 dpf wild-type fish and ubc9 morphants. Arrows indicate the CHT region. (K) Western blotting indicates the expression of free SUMO1s and the SUMO1 conjugates in 6 dpf siblings (left) and tangohkz5/hkz5 mutant embryos (right). (L) Western blotting shows the expression of free SUMO2/3 and the SUMO2/3 conjugates in 6 dpf siblings (left) and tangohkz5/hkz5 mutant embryos (right). (M) Quantification of the SUMO1 conjugates and the free SUMO1 relative to tubulin. (N) Quantification of the SUMO2/3 conjugates and the free SUMO2/3 relative to actin.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
hbae1.1 WT standard conditions 26+ somites intermediate cell mass of mesoderm ISH
WT + MO1-ube2ib standard conditions 26+ somites intermediate cell mass of mesoderm ISH
lyz WT standard conditions 26+ somites myeloid cell ISH
WT + MO1-ube2ib standard conditions 26+ somites myeloid cell ISH
myb WT standard conditions Prim-25 ventral wall of dorsal aorta ISH
Protruding-mouth hematopoietic system posterior side ISH
Day 4 hematopoietic system posterior side ISH
WT + MO1-ube2ib standard conditions Prim-25 ventral wall of dorsal aorta ISH
Protruding-mouth hematopoietic system posterior side ISH
Day 4 hematopoietic system posterior side ISH
sae1 WT standard conditions 1-cell whole organism ISH
Sphere whole organism ISH
75%-epiboly whole organism ISH
14-19 somites brain ISH
14-19 somites whole organism ISH
Prim-5 brain ISH
Prim-5 whole organism ISH
Long-pec brain ISH
Long-pec whole organism ISH
Protruding-mouth brain ISH
Protruding-mouth whole organism ISH
Day 4 whole organism ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-sumo WB WT standard conditions Day 6 whole organism
WB sae1hkz5/hkz5 standard conditions Day 6 whole organism
Ab1-sumo1 WB WT standard conditions Day 6 whole organism
WB sae1hkz5/hkz5 standard conditions Day 6 whole organism
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-ube2ib standard conditions Protruding-mouth blood island hematopoietic stem cell decreased amount, abnormal
Day 4 blood island hematopoietic stem cell decreased amount, abnormal
sae1hkz5/hkz5 standard conditions Day 6 protein sumoylation disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development