FIGURE

Fig. 5

ID

ZDB-FIG-121214-15

Publication

Li et al., 2012 - SUMO1-activating enzyme subunit 1 is essential for the survival of hematopoietic stem/progenitor cells in zebrafish

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Fig. 5

Loss of Sae1 function impairs the sumoylation pathway. (A-D) WISH shows the hbae1 and lyz expression in 23 hpf wild-type fish and ubc9 morphants. Black arrows indicate the ICM. Blue arrows indicate the yolk sac. (E,F) WISH indicates the cmyb expression in 36 hpf wild-type fish and ubc9 morphants. Arrows indicate the AGM of zebrafish. (G-J) WISH shows the cmyb expression in 3-4 dpf wild-type fish and ubc9 morphants. Arrows indicate the CHT region. (K) Western blotting indicates the expression of free SUMO1s and the SUMO1 conjugates in 6 dpf siblings (left) and tango^hkz5^/^hkz5 mutant embryos (right). (L) Western blotting shows the expression of free SUMO2/3 and the SUMO2/3 conjugates in 6 dpf siblings (left) and tango^hkz5^/^hkz5 mutant embryos (right). (M) Quantification of the SUMO1 conjugates and the free SUMO1 relative to tubulin. (N) Quantification of the SUMO2/3 conjugates and the free SUMO2/3 relative to actin.

Expression Data

Genes:	hbae1.1 lyz myb sae1
Antibodies:	Ab1-sumo Ab1-sumo1
Fish:	WT sae1^hkz5/hkz5 WT + MO1-ube2ib
Knockdown Reagent:	MO1-ube2ib
Anatomical Terms:	brain hematopoietic system posterior side intermediate cell mass of mesoderm myeloid cell ventral wall of dorsal aorta whole organism
Stage Range:	1-cell to Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	sae1^hkz5/hkz5 WT + MO1-ube2ib
Knockdown Reagent:	MO1-ube2ib
Observed In:	blood island hematopoietic stem cell protein sumoylation
Stage Range:	Protruding-mouth to Day 6

Phenotype Detail

Acknowledgments

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