Fig. 4

Ajuba and Isl1 Restrict the Number of Isl1-Expressing Cells in the Heart (A) Confocal images of control and Ajuba morphant Tg(myl7:EGFP-HsHRAS)^s883 embryos stained with anti-GFP and anti-Isl1 antibodies at 24 somites. (B and C) Confocal images of control and Ajuba morphant Tg(myl7:EGFP-HsHRAS)^s883 embryos stained with anti-Isl1 antibodies (red) and visualized by GFP fluorescence (green) for myl7 expression at 30 hpf (B) and at 48 hpf (C). (D) Control and zAjuba mRNA-injected Tg(myl7:EGFP-HsHRAS)^s883 embryos at 48 hpf, frontal view (top); in situ hybridization for Isl1 in control or zAjuba mRNA-overexpressing embryos at 48 hpf (bottom). (E) Confocal images of hearts of control, Ajuba, Isl1, and Ajuba/Isl1 morphant Tg(myl7:EGFP-HsHRAS)^s883 embryos, stained with anti-GFP antibody. Arrows show differentiated cardiomyocytes in the pericardial wall adjacent to the heart. (F) In situ hybridization for Isl1 in control, Ajuba, Isl1, and Ajuba/Isl1-deficient embryos. (G) Immunostaining with anti-GFP and in situ hybridization for Isl1 in control (left) and Ajuba/Isl1-deficient Tg(myl7:EGFP-HsHRAS)^s883 (right) embryos from (F). See also Figure S3.