Fig. S5

(related to Figure 5). Isl1 expression in cardiac progenitor cells.

(A, A′, A′′) Expression of the Nkx2.5:GFP transgene in anterior lateral plate mesoderm, adjacent to pharyngeal endoderm, marked by her5:mCherry (Tallafuss and Bally-Cuif, 2003) at different developmental stages. (B) In situ hybridization for Nkx2.5 and immunostaining for GFP (Nkx2.5), showing a similar domain of expression of the Nkx2.5:GFP transgene and the Nkx2.5 mRNA (C, C′, C′′, C′′′). Confocal images of Nkx2.5:GFP transgenic embryos stained with anti-Isl1 and anti-GFP antibodies at the 15 somite stage. C′′ shows the position of the transverse (xz) optical sections presented in C′′′1, 2, 3. (D, D′, D′′, D′′′) Confocal images of Nkx2.5:GFP transgenic embryos stained with anti-Isl1 and anti-GFP antibodies at the 18 somite stage. D′′ shows the position of the transverse optical sections presented in D′′′1, 2, 3. (E, E′, E′′) Confocal images of Nkx2.5:GFP transgenic embryos stained with anti-Isl1 and anti-GFP antibodies at the 21 somite stage. (F, F′, F′′) Confocal images of Tg(myl7:EGFP-HsHRAS)^s883 embryos stained with anti-Isl1 and anti-GFP antibodies at the 24 somite stage. NT, neural tube, Tg, trigeminal placode.