ZFIN ID: ZDB-FIG-120815-25
Mahabaleshwar et al., 2012 - beta-arrestin control of late endosomal sorting facilitates decoy receptor function and chemokine gradient formation. Development (Cambridge, England)   139(16):2897-2902 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: 5-9 somites
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: 5-9 somites

Fig. 4

Role of β-arrestins in discrimination between Cxcl12a expression domains. (A,B) Snapshots from supplementary material Movie 5 (A) and Fig. S6 (B). In early somitogenesis stages, PGCs (asterisks) specified close to the midline migrate laterally (A). In an embryo lacking β-arrestin function (B), this movement is not observed. (C) β-arrestins promote migration of PGCs away from the somites. Red bars (y-axis on left) indicate the average number of cells remaining in the somites; error bars indicate s.e.m. The distribution of the results is indicated by circles (y-axis on right); N, the number of embryos analyzed; **P<0.05, ***P<0.001, Student’s t-test. Bottom panels show examples of in situ hybridizations using nanos1 (nos1; blue, PGCs) and cxcl12a (brown) probes used to generate the graphs. (D) A model for PGC migration during early somitogenesis in wild type (left) and in embryos compromised for β-arrestins (right). The sink function of Cxcr7b (purple), aided by β-arrestins (red ellipsoid), converts uniform cxcl12a mRNA expression into a chemotactic Cxcl12a gradient, directing the PGCs to the right. In the absence of β-arrestins, Cxcr7b function is compromised, increasing Cxcl12a levels in the forming somites and hindering the migration of PGCs towards their target.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cxcl12a AB control 5-9 somites lateral plate mesoderm ISH
5-9 somites somite anterior region ISH
AB + MO1-arrb2a + MO2-arrb1 + MO2-arrb2a + MO2-arrb2b + MO3-arrb2b standard conditions 5-9 somites lateral plate mesoderm ISH
5-9 somites somite anterior region ISH
AB + MO1-arrb2a + MO2-arrb2a standard conditions 5-9 somites lateral plate mesoderm ISH
5-9 somites somite anterior region ISH
AB + MO2-arrb1 standard conditions 5-9 somites lateral plate mesoderm ISH
5-9 somites somite anterior region ISH
AB + MO2-arrb2b + MO3-arrb2b standard conditions 5-9 somites lateral plate mesoderm ISH
5-9 somites somite anterior region ISH
nanos3 AB control 5-9 somites primordial germ cell ISH
AB + MO1-arrb2a + MO2-arrb1 + MO2-arrb2a + MO2-arrb2b + MO3-arrb2b standard conditions 5-9 somites primordial germ cell ISH
AB + MO1-arrb2a + MO2-arrb2a standard conditions 5-9 somites primordial germ cell ISH
AB + MO2-arrb1 standard conditions 5-9 somites primordial germ cell ISH
AB + MO2-arrb2b + MO3-arrb2b standard conditions 5-9 somites primordial germ cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-arrb2a + MO2-arrb1 + MO2-arrb2a + MO2-arrb2b + MO3-arrb2b standard conditions 5-9 somites germ cell migration decreased process quality, abnormal
5-9 somites primordial germ cell mislocalised medially, abnormal
AB + MO1-arrb2a + MO2-arrb2a standard conditions 5-9 somites germ cell migration decreased process quality, abnormal
5-9 somites primordial germ cell mislocalised medially, abnormal
AB + MO2-arrb1 standard conditions 5-9 somites germ cell migration process quality, normal
AB + MO2-arrb2b + MO3-arrb2b standard conditions 5-9 somites germ cell migration decreased process quality, abnormal
5-9 somites primordial germ cell mislocalised medially, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development