ZFIN ID: ZDB-FIG-120810-56
Lee et al., 2012 - Disruption of the folate pathway in zebrafish causes developmental defects. BMC Developmental Biology   12(1):12 Full text @ BMC Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 4-cell to Protruding-mouth

Fig. 2 Wild type expression data for folate pathway transcripts. A) RT-PCR analysis of 11 embryonic stages within the first 72 hours of development indicates that genes involved in the folate pathway are generally maternally loaded transcripts that are expressed throughout early development. B) in situ hybridization for these 16 genes at 24 hpf shows that many are globally expressed with enrichment in anterior neural tissues including tectonic and retinal staining. Specific somatic staining is seen in mat1a, mat2aa, mat2ab, and mthfr. YSL staining is also observed in mat1a and mthfd1b. Embryos in lateral view.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dhfr AB/TU standard conditions 4-cell to Protruding-mouth whole organism RTPCR
Prim-5 anterior neural tube ISH
Prim-5 optic cup ISH
mat1a AB/TU standard conditions 4-cell to Shield Not Detected whole organism RTPCR
Bud to Protruding-mouth whole organism RTPCR
Prim-5 anterior neural tube ISH
Prim-5 optic cup ISH
Prim-5 somite ISH
Prim-5 yolk syncytial layer ISH
mat2aa AB/TU standard conditions 4-cell to Long-pec whole organism RTPCR
Prim-5 anterior neural tube ISH
Prim-5 optic cup ISH
Prim-5 somite ISH
Protruding-mouth Not Detected whole organism RTPCR
mat2ab AB/TU standard conditions 4-cell to Protruding-mouth whole organism RTPCR
Prim-5 anterior neural tube ISH
Prim-5 optic cup ISH
Prim-5 somite ISH
mat2al AB/TU standard conditions 4-cell to Sphere Not Detected whole organism RTPCR
Shield to Protruding-mouth whole organism RTPCR
Prim-5 anterior neural tube ISH
Prim-5 optic cup ISH
mat2b AB/TU standard conditions 4-cell to Protruding-mouth whole organism RTPCR
Prim-5 anterior neural tube ISH
Prim-5 optic cup ISH
mthfd1a AB/TU standard conditions 4-cell to Protruding-mouth whole organism RTPCR
Prim-5 anterior neural tube ISH
Prim-5 optic cup ISH
mthfd1b AB/TU standard conditions 4-cell to Protruding-mouth whole organism RTPCR
Prim-5 anterior neural tube ISH
Prim-5 optic cup ISH
Prim-5 yolk syncytial layer ISH
mthfd1l AB/TU standard conditions 4-cell to Sphere Not Detected whole organism RTPCR
Shield to Protruding-mouth whole organism RTPCR
Prim-5 anterior neural tube ISH
Prim-5 optic cup ISH
mthfd2 AB/TU standard conditions 4-cell to Protruding-mouth whole organism RTPCR
Prim-5 anterior neural tube ISH
Prim-5 optic cup ISH
mthfd2l AB/TU standard conditions 4-cell to Protruding-mouth whole organism RTPCR
Prim-5 anterior neural tube ISH
Prim-5 optic cup ISH
mthfr AB/TU standard conditions 4-cell to Protruding-mouth whole organism RTPCR
Prim-5 anterior neural tube ISH
Prim-5 optic cup ISH
Prim-5 somite ISH
mtr AB/TU standard conditions 4-cell to Protruding-mouth whole organism RTPCR
Prim-5 anterior neural tube ISH
Prim-5 optic cup ISH
slc25a32a AB/TU standard conditions 4-cell to Protruding-mouth whole organism RTPCR
Prim-5 anterior neural tube ISH
Prim-5 optic cup ISH
slc25a32b AB/TU standard conditions 4-cell to Protruding-mouth whole organism RTPCR
Prim-5 anterior neural tube ISH
Prim-5 optic cup ISH
tyms AB/TU standard conditions 4-cell to Protruding-mouth whole organism RTPCR
Prim-5 anterior neural tube ISH
Prim-5 optic cup ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal BMC Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Dev. Biol.