ZFIN ID: ZDB-FIG-120720-41
Huycke et al., 2012 - Hedgehog-dependent proliferation drives modular growth during morphogenesis of a dermal bone. Development (Cambridge, England)   139(13):2371-2380 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Anatomical Term:
Stage: Day 6
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Day 6

Fig. 8 Cyclopamine (CyA) induces vp edge length reduction. Confocal projections of the Op in larvae treated with CyA. Images are lateral views with dorsal upwards and anterior towards the left. (A-C) Wild-type larvae treated with CyA (B) display a reduction in vp edge length and phenocopy ihha mutants (C). (D) Mutants treated with CyA look similar to untreated siblings. (E) Measurements of vj, jp and vp edge lengths for all four treatment categories. Lengths of vj and jp are not statistically different when compared between all possible combinations of treatments. Length of the vp edge is significantly reduced in CyA-treated wild-type larvae compared with wild-type controls, but is not significantly different from ihha mutants treated with CyA or ethanol. Data are presented as meanĀ±s.d., **P<0.01 by Tukey-Kramer test, ne5 per treatment category. vp, edge connecting v and p; vj, edge connecting v and j; jp, edge connecting j and p. Scale bar: 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP b1212Tg control Day 6 unspecified IFL
b1212Tg chemical treatment: Cyclopamine Day 6 unspecified IFL
ihhahu2131/hu2131; b1212Tg control Day 6 unspecified IFL
ihhahu2131/hu2131; b1212Tg chemical treatment: Cyclopamine Day 6 unspecified IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
b1212Tg chemical treatment: Cyclopamine Day 6 opercle postero-ventral margin decreased length, abnormal
ihhahu2131/hu2131; b1212Tg control Day 6 opercle postero-ventral margin decreased length, abnormal
ihhahu2131/hu2131; b1212Tg chemical treatment: Cyclopamine Day 6 opercle postero-ventral margin decreased length, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development