FIGURE

Fig. 5

ID

ZDB-FIG-120626-1

Publication

Eauclaire et al., 2012 - Mutations in vacuolar H(+)-ATPase subunits lead to biliary developmental defects in zebrafish

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Fig. 5

Intrahepatic biliary defects in other V-H⁺-ATPase mutants. (A–D) Right lateral views of 5 dpf larvae after administration of the fluorescent lipid reporter PED-6. (A) Control larva demonstrates normal gallbladder intensity (white arrow). (B) 5 dpf atp6v0d1^-/- mutant, (C) 5 dpf atp6ap1^-/- mutant, and (D) 5 dpf atp6v1a morpholino (MO)-injected larvae all demonstrate a decrease or lack of gallbladder fluorescence (white arrows). (E–H) Confocal projections of cytokeratin immunostaining of 5 dpf liver from control (E), atp6v0d1^-/-, atp6ap1^-/-, and atp6v1a MO-injected larvae. The abnormal cytokeratin pattern in (F–H) has similarities to the pattern noted in pn, with fewer ducts overall and focal areas of ectatic ducts.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	atp6ap1b^{hi112Tg/hi112Tg} atp6v0d1^{hi2188bTg/hi2188bTg} WT + MO2-atp6v1aa WT + MO3-atp6v1aa
Knockdown Reagents:	MO2-atp6v1aa MO3-atp6v1aa
Observed In:	intrahepatic bile duct intrahepatic bile duct development
Stage:	Day 5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 365(2), Eauclaire, S.F., Cui, S., Ma, L., Matous, J., Marlow, F.L., Gupta, T., Burgess, H.A., Abrams, E.W., Kapp, L.D., Granato, M., Mullins, M.C., and Matthews, R.P., Mutations in vacuolar H(+)-ATPase subunits lead to biliary developmental defects in zebrafish, 434-444, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.