Fig. 4

Additional defects in pn larvae. (A–D) Confocal projections of whole-mount cytokeratin immunostaining of livers from 4 dpf wild-type (A, wt) and pekin (B, pn) larvae, demonstrating abnormalities at this earlier stage. Ducts are short and demonstrate few if any connections. These abnormalities are schematized in (C–D). (E–F) Hematoxylin and eosin stainings of cross-sections of 5 dpf wt (E) and pn (F) larvae, demonstrating that at this resolution the liver of a pn larva appears normal. (G–H) Hematoxylin and eosin stainings of the eye from a 5 dpf wt (G) and pn (H) larva. Note the ordered appearance of the cell layers in the wt eye, including the inner ganglionic (ig), outer ganglionic (og), retinal (r), and retinal pigmented epithelial (rpe) layers. In contrast, cells in these layers are disordered in pn, in particular in the retinal layer, which is difficult to distinguish from the og layer. The rpe is also poorly formed in pn. (I–L) Electron micrographs of pancreas (I–J) and enterocytes (K–L) from wt (I, K) and pn (J, L). Note that the presence of numerous zymogen granules (zg) in wt pancreas is absent in pn, although there are small structures that may represent poorly formed zg (black arrow). The Golgi (g) in the pn enterocyte (L) appears dilated relative to the Golgi of wt (K). n, nucleus. Scale bars 500 nm.