FIGURE

Fig. 9

ID

ZDB-FIG-120604-8

Publication

Poulain et al., 2002 - Mezzo, a paired-like homeobox protein is an immediate target of Nodal signalling and regulates endoderm specification in zebrafish

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Fig. 9

Overexpression of mezzo partially rescues heart morphogenesis in bon mutants and increases the number of sox17- and cas-expressing cells. (A-C) Rescue of heart morphogenesis after injection of mezzo mRNA into bon mutant embryos monitored by expression of nkx2.5 at 30 hours. The failure of heart primordia to migrate and fuse in the midline in bon mutants (B) was partially restored by overexpression of mezzo (C). (D-I) Dorsal views of embryos at 80% epiboly, showing expression of endodermal markers in wild-type embryos (D,G), bon mutant embryos (E,H) and bon mutant embryos injected with mezzo mRNA (F,I). Embryos were injected with 20-40 pg of mezzo mRNA a the one- to four-cell stage and examined for expression of sox17 (F) or casanova (I). The number of sox17- or casanova-positive cells is significantly increased in homozygous bon mutants injected with mezzo mRNA (F,I) compared with control uninjected mutant embryos (E,H). bon mutant embryos were genotyped by PCR.

Expression Data

Genes:	nkx2.5 sox17 sox32
Fish:	AB mixl1^m425/m425
Anatomical Terms:	endoderm heart tube
Stage Range:	75%-epiboly to Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mixl1^m425/m425
Observed In:	endodermal cell fate specification heart tube
Stage Range:	75%-epiboly to Prim-15

Phenotype Detail

Acknowledgments

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