header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-120604-3
Poulain et al., 2002 - Mezzo, a paired-like homeobox protein is an immediate target of Nodal signalling and regulates endoderm specification in zebrafish. Development (Cambridge, England)   129(21):4901-4914
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: 30%-epiboly to Shield

Fig. 4

Analyses of mezzo expression in various mutants of the Nodal signalling pathway. All the embryos were fixed at the shield stage except those in B,H (40% epiboly). (A-F) Side views, (G-L) animal pole views. (A,G) Control wild-type embryos. Expression of mezzo was lost on the dorsal side in Zoep (B,H) and squint (C,I) mutant embryos. Expression of mezzo was abolished in cyc;sqt double mutants (D,J) and variably affected in MZsur embryos. The variable expression of mezzo in MZsur correlated with the expressivity of the schmalspur phenotype. The embryos shown in E,K derived from a cross producing severely affected embryos. Expression of mezzo is greatly diminished in two thirds of the marginal region in bon;faust double mutants (F,L).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
sebox AB standard conditions Shield margin ISH
foxh1m768/m768 standard conditions Shield margin ISH
Shield Not Detected margin dorsal-most region ISH
gata5tm236a/tm236a; mixl1m425/m425 standard conditions Shield margin ventral side ISH
ndr1cz35/cz35 standard conditions Shield margin ISH
Shield Not Detected margin dorsal-most region ISH
ndr1cz35/cz35; ndr2Df(Chr12:hhex)b16/b16 standard conditions Shield Not Detected margin ISH
tdgf1m134/m134 standard conditions 30%-epiboly margin ISH
30%-epiboly Not Detected margin dorsal-most region ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright.