ZFIN ID: ZDB-FIG-120601-82
van der Velden et al., 2012 - The Polycomb group protein Ring1b is essential for pectoral fin development. Development (Cambridge, England)   139(12):2210-2220 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec

Fig. S2 Hox gene expression in ring1b mutants. (A-X) Lateral views of in situ hybridization for hoxa9b (A,B,I,J,Q,R), hoxc6a (C,D,K,L,S,T), hoxc8a (E,F,M,N,U,V) and hoxd9a (G,H,O,P,W,X) at 24 hpf (A-H), 48 hpf (I-P) and 72 hpf (Q-X). Axial Hox gene expression is anteriorly restricted in wild-type larvae (A,C,E,G), whereas the anterior border of axial Hox gene expression is slightly less defined in ring1b mutants at 24 hpf (B,D,F,H) (arrowheads). Ectopic Hox gene expression is progressively upregulated in ring1b mutants (B,D,F,H versus J,L,N,P and R,T,V,X). Strong ectopic expression throughout the brain is observed especially for hoxa9b (R), hoxc6a (T) and hoxc8a (V) in ring1b mutants at 72 hpf. Also note that Hox gene expression is absent or severely reduced in the pectoral fin field of ring1b mutants.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
hoxa9b WT standard conditions Prim-5 head ISH
Prim-5 whole organism postero-dorsal region ISH
Prim-15 head ISH
Prim-15 pectoral fin bud ISH
Prim-15 whole organism postero-dorsal region ISH
Long-pec pectoral fin ISH
Long-pec whole organism postero-dorsal region ISH
rnf2ibl30/ibl30 standard conditions Prim-5 head ISH
Prim-5 whole organism postero-dorsal region ISH
Prim-15 head ISH
Prim-15 whole organism dorsal region ISH
Long-pec head ISH
Long-pec whole organism dorsal region ISH
rnf2ibl31/ibl31 standard conditions Prim-5 head ISH
Prim-5 whole organism postero-dorsal region ISH
Prim-15 head ISH
Prim-15 whole organism dorsal region ISH
Long-pec head ISH
Long-pec whole organism dorsal region ISH
hoxc6a WT standard conditions Prim-5 pectoral fin bud ISH
Prim-5 whole organism dorsal region ISH
Prim-15 pectoral fin bud ISH
rnf2ibl30/ibl30 standard conditions Prim-5 whole organism dorsal region ISH
Prim-15 whole organism dorsal region ISH
Long-pec brain ISH
rnf2ibl31/ibl31 standard conditions Prim-5 whole organism dorsal region ISH
Prim-15 whole organism dorsal region ISH
Long-pec brain ISH
hoxc8a WT standard conditions Prim-5 whole organism dorsal region ISH
Prim-15 whole organism dorsal region ISH
rnf2ibl30/ibl30 standard conditions Prim-5 whole organism dorsal region ISH
Prim-15 whole organism dorsal region ISH
Long-pec brain ISH
Long-pec whole organism dorsal region ISH
rnf2ibl31/ibl31 standard conditions Prim-5 whole organism dorsal region ISH
Prim-15 whole organism dorsal region ISH
Long-pec brain ISH
Long-pec whole organism dorsal region ISH
hoxd9a WT standard conditions Prim-5 pectoral fin bud ISH
Prim-5 whole organism postero-dorsal region ISH
Prim-15 pectoral fin bud ISH
Prim-15 whole organism postero-dorsal region ISH
Long-pec pectoral fin ISH
Long-pec whole organism postero-dorsal region ISH
rnf2ibl30/ibl30 standard conditions Prim-5 whole organism postero-dorsal region ISH
Prim-15 whole organism dorsal region ISH
Long-pec whole organism dorsal region ISH
rnf2ibl31/ibl31 standard conditions Prim-5 whole organism postero-dorsal region ISH
Prim-15 whole organism dorsal region ISH
Long-pec whole organism dorsal region ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development