|
Fig. S2 Hox gene expression in ring1b mutants. (A-X) Lateral views of in situ hybridization for hoxa9b (A,B,I,J,Q,R), hoxc6a (C,D,K,L,S,T), hoxc8a (E,F,M,N,U,V) and hoxd9a (G,H,O,P,W,X) at 24 hpf (A-H), 48 hpf (I-P) and 72 hpf (Q-X). Axial Hox gene expression is anteriorly restricted in wild-type larvae (A,C,E,G), whereas the anterior border of axial Hox gene expression is slightly less defined in ring1b mutants at 24 hpf (B,D,F,H) (arrowheads). Ectopic Hox gene expression is progressively upregulated in ring1b mutants (B,D,F,H versus J,L,N,P and R,T,V,X). Strong ectopic expression throughout the brain is observed especially for hoxa9b (R), hoxc6a (T) and hoxc8a (V) in ring1b mutants at 72 hpf. Also note that Hox gene expression is absent or severely reduced in the pectoral fin field of ring1b mutants.