FIGURE

Fig. S8

ID

ZDB-FIG-120601-88

Publication

van der Velden et al., 2012 - The Polycomb group protein Ring1b is essential for pectoral fin development

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Fig. S8

Impaired AP/DV patterning and loss of AER gene expression in ring1b mutants. (A) Dorsal view of in situ hybridization for the AP markers msxc (a,b) and shh (c,d) and the DV markers eng1a (e,f) and wnt7a (g,h) at 32 hpf. msxc is not restricted to the anterior pectoral fin mesenchyme because shh is not expressed in the zone of polarizing activity (ZPA) of ring1b mutants (b,d). eng1a expression is greatly reduced, whereas wnt7a is not expressed in the ring1b pectoral fin ectoderm (f,h). (B) Dorsal (a-h) and lateral (a′-h′) views of in situ hybridizations for the AER markers dlx2a (a-b′), fgf8 (c-d′), fgf24 (e-f′) and versican (g-h′) at 40 hpf. The examined AER markers are not expressed in the pectoral fin ectoderm of ring1b mutants at 40 hpf and fin bud outgrowth is not initiated (b-h′).

Expression Data

Gene:	wnt7aa
Fish:	WT rnf2^ibl30/ibl30 rnf2^ibl31/ibl31
Anatomical Terms:	apical ectodermal ridge pectoral fin bud head
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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