Fig. 6

hb9 expression is initiated but not maintained in Pax6b deficient embryos. (A–F) Expression of hb9 mRNA at 24 hpf in motoneurons (MN in A, C, E) and in the pancreatic islet (pancreas in B, D, F) of uninjected wild type (wt) embryos (A, B) and embryos injected with neuroD (C, D) or pax6b morpholinos (E, F). Note that pancreatic hb9 expression is reduced in pax6b but not in neuroD morphants while motoneuron specific expression in the same embryos is not affected. Embryos are shown in dorsal view with anterior to the left. Scale bars in all figures correspond to 20 µm. (G) Number of hb9 positive cells in the pancreas of the wild type (wt), neuroD (MO^neuroD) and pax6b (MO^pax6b) morphants. (p-value for neuroD morphants 0.19429, p-value for pax6b morphants 0.00467). (H–O) hb9 expression in wild type embryos (H, I, J, K) and pax6b mutants (L, M, N, O) at 18 hpf (H, L), 25 hpf (I, M), 30 hpf (J, N) and 35 hpf (K, O). Note that hb9 expression in pancreas (white arrowhead) decreases during development in mutants, whereas expression in motoneurons (white arrow) is not affected. Embryos are shown in lateral view with anterior to the left. Scale bars in all figures correspond to 20 μm.