ZFIN ID: ZDB-FIG-120423-24
Arkhipova et al., 2012 - Characterization and regulation of the hb9/mnx1 beta-cell progenitor specific enhancer in zebrafish. Developmental Biology   365(1):290-302 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Anatomical Term:
Stage: Prim-5

Fig. 3

478 bp of proximal hb9 promoter sequences are sufficient for beta-cell specific regulation. (A) Overview of transient GFP reporter experiments with a scheme of deletion constructs on the left and corresponding numbers of GFP positive embryos (n) and embryos expressing GFP in the pancreas (P) on the right side. (B–F) GFP expression in representative transgenic lines at 28 hpf in whole embryos (left panel) and in fixed tissue (right panel) stained with antibodies for GFP (green) and insulin (blue). The right panel shows confocal image projections and the corresponding overlays (right-most panel). Scale bar corresponds to 100 μm (whole embryos) and 20 μm (confocal image stacks). For more details see also Supplementary Table 2.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP Ab3-GFP m1018Tg; ml6Tg standard conditions Prim-5 pancreas primordium IHC
Ab3-GFP m1018Tg; ml7Tg standard conditions Prim-5 pancreas primordium IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab3-ins IHC m1018Tg; ml6Tg standard conditions Prim-5 pancreas primordium
IHC m1018Tg; ml7Tg standard conditions Prim-5 pancreas primordium
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 365(1), Arkhipova, V., Wendik, B., Devos, N., Ek, O., Peers, B., and Meyer, D., Characterization and regulation of the hb9/mnx1 beta-cell progenitor specific enhancer in zebrafish, 290-302, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.