FIGURE

Fig. 2

ID

ZDB-FIG-120316-17

Publication

Zannino et al., 2012 - Olig2-expressing hindbrain cells are required for migrating facial motor neurons

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Facial motor neurons fail to completely exit r4 and r5 to form clusters in r6 and r7. Panels show a single z-plane image of dorsally mounted whole embryos with anterior to the left. Tg(isl1:GFP)^rw0 reporter expression, amplified using an anti-GFP antibody and an Alexa 488 secondary antibody, is revealed as green and RNA expression patterns are blue. A,B:hoxb1a expression marks r4. C–F:egr2b expression labels r3 and r5. G–L:hoxb4a expression begins at the r6/r7 boundary and extends posteriorly. Arrows indicate region where facial motor neurons normally migrate. Approximate location of the otic vesicles is outlined with red ovals. Scale bar = 32 μm.

Expression Data

Genes:	egr2b GFP hoxb1a hoxb4a
Fish:	rw0Tg rw0Tg + MO4-olig2
Knockdown Reagent:	MO4-olig2
Anatomical Terms:	facial nerve motor nucleus motor neuron rhombomere 3 rhombomere 4 rhombomere 5 rhombomere 7
Stage Range:	20-25 somites to Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	rw0Tg + MO4-olig2
Knockdown Reagent:	MO4-olig2
Observed In:	facial nerve motor nucleus motor neuron neuron migration
Stage Range:	20-25 somites to Prim-15

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.