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ZIRC
ZFIN ID: ZDB-FIG-120216-84
Wu et al., 2011 - Ryanodine receptors, a family of intracellular calcium ion channels, are expressed throughout early vertebrate development. BMC research notes   4(1):541 Full text @ BMC Res. Notes
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 4-cell to Adult

Fig. 3

Zebrafish ryr mRNA can be detected from very early developmental stages through to adulthood. (a) Temporal expression pattern of zebrafish ryrs using end-point PCR analysis and isoform specific primers. Total RNA was isolated from different developmental stages, as indicated, and reverse transcribed. 1.5 μg of cDNA was synthesised and isoform specific primers for ryr1a, ryr1b, ryr2a, ryr2b and ryr3 used. Numbers in the right panel indicate the molecular mass of PCR products. β-actin was used as an internal control. The figure shows a representative result of replicates from at least three experiments. (b) Expression of zebrafish ryr2a and ryr2b in the brain (BR) and body (BO) of a 3 months old adult zebrafish. β-actin was used as an internal control. Numbers in the right panel indicate the molecular mass of RT-PCR products. (c) Maternal expression of ryr3 mRNA was observed as staining (arrows) within the dividing cells of 2-2.25 hpf embryos (64 and 128-cell stage). The specificity of the signal is compared to the ryr3 sense probes.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
ryr1a WT standard conditions 4-cell to 128-cell Not Detected whole organism RTPCR
50%-epiboly Not Detected whole organism RTPCR
Bud Not Detected whole organism RTPCR
1-4 somites to 5-9 somites whole organism RTPCR
14-19 somites whole organism RTPCR
Prim-5 whole organism RTPCR
Protruding-mouth whole organism RTPCR
Day 6 whole organism RTPCR
Adult whole organism RTPCR
ryr1b WT standard conditions 4-cell to 128-cell Not Detected whole organism RTPCR
50%-epiboly Not Detected whole organism RTPCR
Bud Not Detected whole organism RTPCR
1-4 somites to 5-9 somites Not Detected whole organism RTPCR
14-19 somites whole organism RTPCR
Prim-5 whole organism RTPCR
Protruding-mouth whole organism RTPCR
Day 6 whole organism RTPCR
Adult whole organism RTPCR
ryr2a WT standard conditions 4-cell to 128-cell whole organism RTPCR
50%-epiboly Not Detected whole organism RTPCR
Bud Not Detected whole organism RTPCR
1-4 somites to 5-9 somites Not Detected whole organism RTPCR
14-19 somites whole organism RTPCR
Prim-5 whole organism RTPCR
Protruding-mouth whole organism RTPCR
Day 6 whole organism RTPCR
Adult brain RTPCR
Adult whole organism RTPCR
ryr2b WT standard conditions 4-cell to 128-cell Not Detected whole organism RTPCR
50%-epiboly Not Detected whole organism RTPCR
Bud Not Detected whole organism RTPCR
14-19 somites whole organism RTPCR
Prim-5 whole organism RTPCR
Protruding-mouth whole organism RTPCR
Day 6 whole organism RTPCR
Adult whole organism RTPCR
ryr3 WT standard conditions 4-cell to 128-cell whole organism RTPCR
128-cell whole organism ISH
64-cell whole organism ISH
50%-epiboly whole organism RTPCR
1-4 somites to 5-9 somites whole organism RTPCR
14-19 somites whole organism RTPCR
Prim-5 whole organism RTPCR
Protruding-mouth whole organism RTPCR
Day 6 whole organism RTPCR
Adult whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal BMC research notes for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Res. Notes