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ZIRC
ZFIN ID: ZDB-FIG-120216-85
Wu et al., 2011 - Ryanodine receptors, a family of intracellular calcium ion channels, are expressed throughout early vertebrate development. BMC research notes   4(1):541 Full text @ BMC Res. Notes
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage Range: 1-4 somites to Prim-5

Fig. 4

Zebrafish ryr1a, ryr1b and ryr3 mRNA is localised to embryonic skeletal muscle. (a) Expression of zebrafish myoD, ryr1a, ryr1b and ryr3 was examined using whole mount in situ hybridisation. Expression of ryr1a mRNA is detectable in the adaxial cells of 11 hpf embryos (3-somite, arrows), whereas ryr1b mRNA expression is present in cells adjacent to the notochord from 12 hpf (6-somite stage) embryos. Expression of ryr3 mRNA was only evident at 24 hpf, with the strongest staining observed in the anterior somites. The embryos at 11 and 12 hpf are orientated so that the anterior is to the left. (b) Cross-sections showing myoD, ryr1a, ryr1b and ryr3 in-situ mRNA hybridisation (above) and double immunostained with the F59 antibody/HRP labeling (below) in 24 hpf embryos. (c) ryr1a, ryr1b and ryr3 mRNA expression in wildtype (smo+/+), heterozygote (smo+/-) and homozygous (smo-/-) mutants at 24 hpf. There is no ryr1a mRNA expression in the smo-/- mutant, compared to wildtype and heterozygous embryos at 24 hpf.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
ryr1a WT standard conditions 1-4 somites adaxial cell ISH
5-9 somites somite ISH
10-13 somites somite ISH
14-19 somites somite ISH
Prim-5 slow muscle cell ISH
smounspecified/unspecified standard conditions Prim-5 Not Detected whole organism ISH
ryr1b WT standard conditions 1-4 somites Not Detected whole organism ISH
5-9 somites adaxial cell ISH
10-13 somites somite ISH
14-19 somites somite ISH
Prim-5 fast muscle cell ISH
Prim-5 slow muscle cell ISH
Prim-5 somite ISH
smounspecified/unspecified standard conditions Prim-5 somite ISH
ryr3 WT standard conditions 1-4 somites Not Detected whole organism ISH
5-9 somites Not Detected whole organism ISH
10-13 somites Not Detected whole organism ISH
14-19 somites Not Detected whole organism ISH
Prim-5 fast muscle cell ISH
Prim-5 somite ISH
smounspecified/unspecified standard conditions Prim-5 somite ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-F59 IHC WT standard conditions Prim-5 slow muscle cell
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal BMC research notes for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Res. Notes