FIGURE

Fig. S2

ID

ZDB-FIG-120216-70

Publication

Yoruk et al., 2012 - Ccm3 functions in a manner distinct from Ccm1 and Ccm2 in a zebrafish model of CCM vascular disease

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Fig. S2

Cardiovascular defects caused by morpholino-knockdown of ccm3a/b can be rescued by overexpression of ccm3b mRNA. (A) Light images of ccm3b mRNA injected and non-injected ccm3a/b morphant embryos at 54 hpf. The pronounced pericardial edema and circulatory block observed in 93% of the embryos upon 0.25 ng ccm3a/b morpholino injection (n = 83) are rescued by co-injection of 400 pg ccm3b mRNA. 63% of ccm3a/b morphant embryos injected with ccm3b mRNA had normal heart morphology and completely restored circulation (n = 76). (B) Confocal z-stack projections of dorsal head vasculature in ccm3a/b morphants indicate a decrease in the dilation and misconnections in cranial vessels upon ccm3b mRNA injection (yellow arrows).

Expression Data

Gene:	EGFP
Fish:	s843Tg s843Tg + MO1-pdcd10a,pdcd10b
Knockdown Reagent:	MO1-pdcd10a,pdcd10b
Anatomical Term:	cranial vasculature
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-pdcd10a,pdcd10b s843Tg + MO1-pdcd10a,pdcd10b
Knockdown Reagent:	MO1-pdcd10a,pdcd10b
Observed In:	blood circulation cranial vasculature pericardium
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 362(2), Yoruk, B., Gillers, B.S., Chi, N.C., and Scott, I.C., Ccm3 functions in a manner distinct from Ccm1 and Ccm2 in a zebrafish model of CCM vascular disease, 121-131, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.